Fibrin beta-chain Antibody (8E5) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: ELISA, Flow, Func
• Clone: 8E5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

Fibrin beta-chain Antibody (8E5) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: 8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic beta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).
• Specificity: 8E5 binds to the amino-terminal region of the beta-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. This peptide sequence is found only in fibrin, and not in its precursor fibrinogen and so there is no cross-reactivity with fibrinogen. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FGB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • In vivo assay
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Fibrin beta-chain Antibody (8E5) [Alexa Fluor® 532]

• Beta-Fibrinogen
• epididymis secretory sperm binding protein Li 78p
• fibrinogen beta chain
• fibrinogen, B beta polypeptide
• HEL-S-78p

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGB