Fibrin beta-chain Antibody (8E5) [Alexa Fluor™ Plus 555] - Chimeric

• Reactivity: Hu
• Applications: ELISA, Flow, Func
• Clone: 8E5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 555

Fibrin beta-chain Antibody (8E5) [Alexa Fluor™ Plus 555] - Chimeric Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: 8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic beta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).
• Specificity: 8E5 binds to the amino-terminal region of the beta-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. This peptide sequence is found only in fibrin, and not in its precursor fibrinogen and so there is no cross-reactivity with fibrinogen. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FGB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • In vivo assay
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Fibrin beta-chain Antibody (8E5) [Alexa Fluor™ Plus 555] - Chimeric

• Beta-Fibrinogen
• epididymis secretory sperm binding protein Li 78p
• fibrinogen beta chain
• fibrinogen, B beta polypeptide
• HEL-S-78p

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGB