FGFR3 Antibody (136334) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
136334
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

FGFR3 Antibody (136334) [Unconjugated] Summary

Additional Information
Clone 136334 was used by HLDA to establish CD designation
Immunogen
Mouse myeloma cell line NS0- and Sf21-derived recombinant human FGF R3 alpha (IIIb)
Specificity
Detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots. Does not cross-react with any isoforms of recombinant mouse (rm) FGF R3, rmFGF R2, recombinant human (rh) FGF R1, rhFGF R2, or rhFGF R4.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Control
FGFR3 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
MAB766 in the following applications:

Publications
Read Publications using
MAB766 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR3 Antibody (136334) [Unconjugated]

  • CD333
  • CEK
  • EC 2.7.10
  • FGF R3
  • FGFR3
  • fibroblast growth factor receptor 3
  • HSFGFR3EX
  • JTK4
  • JTK4thanatophoric dwarfism

Background

Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co‑factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF -1, -2, -4, -8b, -8e, -8f, -9, and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGFR3 Antibody (MAB766)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


Filter By Application
Flow Cytometry
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(3)
All Species

Review for FGFR3 Antibody (MAB766) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB766:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry FGFR3 MAB766
Enlarge
reviewed by:
Natalie Erdmann
ICC Human 01/28/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedKMS11, KMS18 and RPMI8226 (multiple myeloma) cell lines
SpeciesHuman
LotGGC081601A

Blocking

Blocking Details10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

Dilution Ratio1/50 1ary antibody diluted in 10% donkey serum/PBS overnight at 4oC

Secondary Antibody

Secondary DescriptionDonkey F(ab')2 anti-mouse IgG (H+L) Alexa Fluor 488
Secondary Manufacturer Cat#Jackson 711-546-150
Secondary Concentration1/1000

Details

Detection NotesZeiss AxioImager, FITC filter cube for 300 ms exposure
Fixation DetailsImmersion-fixed in 2% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
Wash Description3X 5 minutes PBS after each step

Comments

CommentsPositive controls KMS11 (FGFR3 IIIc) and KMS18 (FGFR3 IIIb); negative control RPMI8226

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FGFR3 Antibody (MAB766) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Natalie Erdmann
01/28/2016
Application: ICC
Species: Human

Bioinformatics

Gene Symbol FGFR3