FGFR3 Antibody (136334) [Unconjugated]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 136334
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FGFR3 Antibody (136334) [Unconjugated] Summary

• Additional Information: Clone 136334 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0- and Sf21-derived recombinant human FGF R3 alpha (IIIb)
• Specificity: Detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots. Does not cross-react with any isoforms of recombinant mouse (rm) FGF R3, rmFGF R2, recombinant human (rh) FGF R1, rhFGF R2, or rhFGF R4.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR3

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
• Control:

  FGFR3 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR3 Antibody (136334) [Unconjugated]

• CD333
• CEK
• EC 2.7.10
• FGF R3
• FGFR3
• fibroblast growth factor receptor 3
• HSFGFR3EX
• JTK4
• JTK4thanatophoric dwarfism

Background

Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co‑factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF -1, -2, -4, -8b, -8e, -8f, -9, and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR3 Antibody (MAB766)(3)

Publications using MAB766

• BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma Sci Rep, 2019;9(1):4902. 2019 [PMID: 30894629] (Flow Cytometry, Human)
• R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner Tumor-associated B-cells induce tumor heterogeneity and therapy resistance Nat Commun, 2017;8(1):607. 2017 [PMID: 28928360] (Western Blot, Human)
• Stewart AK, Chang H, Trudel S Diagnostic evaluation of t(4;14) in multiple myeloma and evidence for clonal evolution. Leukemia, 2007;21(11):2358-9. 2007 [PMID: 17568814] (Flow Cytometry, Human)

Review for FGFR3 Antibody (MAB766) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Natalie Erdmann ICC Human 01/28/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: KMS11, KMS18 and RPMI8226 (multiple myeloma) cell lines
• Species: Human
• Lot: GGC081601A

Blocking

• Blocking Details: 10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: 1/50 1ary antibody diluted in 10% donkey serum/PBS overnight at 4oC

Secondary Antibody

• Secondary Description: Donkey F(ab')2 anti-mouse IgG (H+L) Alexa Fluor 488
• Secondary Manufacturer Cat#: Jackson 711-546-150
• Secondary Concentration: 1/1000

Details

• Detection Notes: Zeiss AxioImager, FITC filter cube for 300 ms exposure
• Fixation Details: Immersion-fixed in 2% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
• Wash Description: 3X 5 minutes PBS after each step

Comments

• Comments: Positive controls KMS11 (FGFR3 IIIc) and KMS18 (FGFR3 IIIb); negative control RPMI8226

Bioinformatics

• Gene Symbol: FGFR3