FGFR3 Antibody (136318) [Unconjugated]

Images

 
Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clone
136318
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

FGFR3 Antibody (136318) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIc)
Specificity
Detects the IIIb and IIIc isoforms of human FGF R3. It does not cross-react with mouse FGF R3 or with any isoforms of recombinant human (rh) FGF R1 or rhFGF R2.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR3
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Neutralization 2.5-15 ng/mL
Control
FGFR3 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
MAB7661 in the following applications:

Publications
Read Publications using
MAB7661 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR3 Antibody (136318) [Unconjugated]

  • CD333
  • CEK
  • EC 2.7.10
  • FGF R3
  • FGFR3
  • fibroblast growth factor receptor 3
  • HSFGFR3EX
  • JTK4
  • JTK4thanatophoric dwarfism

Background

The extracellular domain of FGF R3 includes 3 Ig-like domains. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms.


Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGFR3 Antibody (MAB7661)(2)

Review for FGFR3 Antibody (MAB7661) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB7661:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry FGFR3 MAB7661
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reviewed by:
Natalie Erdmann
ICC Human 01/28/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedKMS11, KMS18 and RPMI8226 (multiple myeloma) cell lines
SpeciesHuman
LotGHV021601A

Blocking

Blocking Details10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

Dilution Ratio1/50 1ary antibody diluted in 10% donkey serum/PBS overnight at 4oC

Secondary Antibody

Secondary DescriptionDonkey F(ab')2 anti-mouse IgG (H+L) Alexa Fluor 488
Secondary Manufacturer Cat#Jackson 711-546-150
Secondary Concentration1/1000

Details

Detection NotesZeiss AxioImager, FITC filter cube for 300 ms exposure
Fixation DetailsImmersion-fixed in 2% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
Wash Description3X 5 minutes PBS after each step

Comments

CommentsPositive controls KMS11 (FGFR3 IIIc) and KMS18 (FGFR3 IIIb), negative control RPMI8226.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FGFR3 Antibody (MAB7661) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Natalie Erdmann
01/28/2016
Application: ICC
Species: Human

Bioinformatics

Gene Symbol FGFR3