FGFR3 Antibody (136318) [Unconjugated]

Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIc) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R3 Monoclonal Antibody (Catalog # MAB7661). The ND50 is typically 2.5-15 ng/mL in the presence of heparin (10 µg/mL).

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 136318
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FGFR3 Antibody (136318) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIc)
• Specificity: Detects the IIIb and IIIc isoforms of human FGF R3. It does not cross-react with mouse FGF R3 or with any isoforms of recombinant human (rh) FGF R1 or rhFGF R2.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR3
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Neutralization 2.5-15 ng/mL
• Control:

  FGFR3 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR3 Antibody (136318) [Unconjugated]

• CD333
• CEK
• EC 2.7.10
• FGF R3
• FGFR3
• fibroblast growth factor receptor 3
• HSFGFR3EX
• JTK4
• JTK4thanatophoric dwarfism

Background

The extracellular domain of FGF R3 includes 3 Ig-like domains. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR3 Antibody (MAB7661)(2)

Publications using MAB7661

• Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. Stem Cells, 2012;30(7):1455-64. 2012 [PMID: 22495904] (Neutralization, Human)
• Shin JW, Min M, Larrieu-Lahargue F, Canron X, Kunstfeld R, Nguyen L, Henderson JE, Bikfalvi A, Detmar M, Hong YK Prox1 promotes lineage-specific expression of fibroblast growth factor (FGF) receptor-3 in lymphatic endothelium: a role for FGF signaling in lymphangiogenesis. Mol. Biol. Cell, 2005;17(2):576-84. 2005 [PMID: 16291864] (IHC-Fr, Human)

Review for FGFR3 Antibody (MAB7661) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Natalie Erdmann ICC Human 01/28/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: KMS11, KMS18 and RPMI8226 (multiple myeloma) cell lines
• Species: Human
• Lot: GHV021601A

Blocking

• Blocking Details: 10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: 1/50 1ary antibody diluted in 10% donkey serum/PBS overnight at 4oC

Secondary Antibody

• Secondary Description: Donkey F(ab')2 anti-mouse IgG (H+L) Alexa Fluor 488
• Secondary Manufacturer Cat#: Jackson 711-546-150
• Secondary Concentration: 1/1000

Details

• Detection Notes: Zeiss AxioImager, FITC filter cube for 300 ms exposure
• Fixation Details: Immersion-fixed in 2% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
• Wash Description: 3X 5 minutes PBS after each step

Comments

• Comments: Positive controls KMS11 (FGFR3 IIIc) and KMS18 (FGFR3 IIIb), negative control RPMI8226.

Bioinformatics

• Gene Symbol: FGFR3