FGF-23 Antibody (275802) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 275802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FGF-23 Antibody (275802) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FGF-23
  Tyr25-Ile251 (Arg179Gln)
  Accession # Q9GZV9
• Specificity: Detects human FGF-23 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, -21, recombinant mouse FGF-8b, -8c, or -15.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF23
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-23 Antibody (275802) [Unconjugated]

• ADHR
• FGF23
• FGF-23
• fibroblast growth factor 23
• HPDR2
• HYPF
• phosphatonin
• PHPTC
• tumor-derived hypophosphatemia inducing factor
• Tumor-derived hypophosphatemia-inducing factor

Background

Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF family, within a subfamily that also includes FGF-19 and FGF-21. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). FGF-19 subfamily members are highly diffusible molecules owing to their poor ECM/heparin sulfate binding and plasma-stabilizing intramolecular folds (2-4). Mature human FGF-23 contains an atypical (very low affinity) heparin binding site (aa 134-162), a proteolytic cleavage site (Arg179-Ser180), and multiple O-linked glycosylation sites with Thr178 being of particular importance (4-7). O-linked glycosylation at Thr178 blocks the cleavage of FGF-23, thereby preventing loss of FGF-23 activity (7, 8). Mature human FGF-23 shows 72% aa identity to mouse FGF-23 and is active on mouse cells (6). FGF-23 exerts its effects through a ternary complex that includes Klotho and an FGF receptor (FGF R4 or the "c" isoforms of FGF R1 or FGF R3). Klotho has a restricted distribution that limits FGF-23 activity (9-11). FGF-23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading. It functions as an endocrine phosphatonin by suppressing circulating phosphate levels (12). FGF-23 interaction with renal proximal tubular epithelium decreases the renal resorption of phosphate by downregulating phosphate transporters and by suppressing vitamin D production. It also decreases the intestinal absorption of phosphate (13).

1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
2. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
3. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
4. Harmer, N.J. et al. (2004) Biochemistry 43:629.
5. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
6. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
7. Frishberg, Y. et al. (2007) J. Bone Miner. Res. 22:235.
8. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
9. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
10. Urakawa, I. et al. (2006) Nature 444:770.
11. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
12. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
13. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF23
• Uniprot:
  • Q9GZV9()