FGF-23 Antibodies

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="FGF-23 was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse FGF-23 Monoclonal Antibody (Catalog # MAB26291) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

1819-KL) and heparin (10 µg/mL), Recombinant Human FGF-23 (Catalog # 2604-FG) stimulates proliferation in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-23 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2604). The ND₅₀ is typically 5-15 µg/mL." class="big_lightbox"> 1819-KL) and heparin (10 µg/mL), Recombinant Human FGF-23 (Catalog # 2604-FG) stimulates proliferation in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-23 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2604). The ND₅₀ is typically 5-15 µg/mL." alt="In the presence of Recombinant Mouse Klotho (5 µg/mL, Catalog # 1819-KL) and heparin (10 µg/mL), Recombinant Human FGF-23 (Catalog # 2604-FG) stimulates proliferation in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-23 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2604). The ND₅₀ is typically 5-15 µg/mL." class="big_thumb" />

HAF019). A specific band was detected for FGF-23 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF019). A specific band was detected for FGF-23 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of bEnd.3 mouse endothelioma cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Mouse FGF-23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2629) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF-23 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />