FGF-22 Antibody (435008)

Recombinant Human FGF‑22 (Catalog # 3867-FG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose‑dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑22 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF‑22 Monoclonal Antibody (Catalog # MAB3867). The ND50 is typically 1.5‑7.5 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 435008
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FGF-22 Antibody (435008) Summary

• Immunogen: E. coli-derived recombinant human FGF-22
  Thr23-Ser170
  Accession # Q9HCT0
• Specificity: Detects human FGF-22 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FGF-basic, rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, -21, -23, recombinant mouse (rm) FGF-basic, rmFGF-8C, or -15 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF22
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 1.5-7.5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-22 Antibody (435008)

• FGF22
• FGF-22
• fibroblast growth factor 22

Background

Fibroblast growth factor-22 (FGF-22) is a 23 kDa, non-glycosylated member of the FGF-7 subfamily, from the FGF family of heparin-binding growth factors (1‑3). The human FGF-22 precursor is 170 amino acids (aa) in length, and contains a 22 aa signal sequence with a 148 aa mature region (4‑6). The mature region shows a centrally-placed, 120 aa beta -trefoil region (aa 43‑168) that is characteristic of all FGF family members. Human FGF-22 potentially has one alternate splice form. This isoform is 129 aa in length, and shows a 31 aa substitution for the first N-terminal 72 aa of the standard, or long, form (7). There is no information related to its possible function. Mature human FGF-22 is 86% aa identical to mouse FGF-22, with the mouse molecule showing a 9 aa deletion at the N-terminus (5). FGF-22 is synthesized by at least three cell types; keratinocytes, neurons, and skeletal muscle myotubes (4, 8, 9). In neurons and myotubes, FGF-22 is presumed to function as an organizer of the presynaptic apparatus. Expressed by postsynaptic (or target) cells, FGF-22 is believed to bind to FGF R2b on the surface of innervating processes, resulting in synaptic vesicle clustering, organization, and neurite branching (8, 10). Although FGF-22 is assumed to be secreted, little can be found in expressing cell culture media. Presumably, it is bound to 34 kDa FGF-BP1, which is a molecule described as typically associated with cell membrane proteoglycans (6, 11). Thus, following secretion, FGF-22 could quickly be immobilized by FGF-BP1, only to be released at a later time, or aided by FGF-BP1 in its interaction with FGF R2b (6, 10, 11).

1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
2. Ornitz, D.M. and N. Itoh (2001) Genome Biol. 2:3005.1 Epub 2001 Mar 9.
3. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
4. Beyer, T.A. et al. (2003) Exp. Cell Res. 287:228.
5. Nakatake, Y. et al. (2001) Biochim. Biophys. Acta 1517:460.
6. Beer, H-D. et al. (2005) Oncogene 24:5269.
7. GenBank Accession # EAW61176.
8. Fox, M.A. and H. Umemori (2006) J. Neurochem. 97:1215.
9. Umemori, H. et al. (2004) Cell 118:257.
10. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
11. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF22
• Uniprot:
  • Q9HCT0()