Rat Ferritin Heavy Chain ELISA Kit (Colorimetric)

ELISA: Rat Ferritin Heavy Chain ELISA Kit (Colorimetric) [NBP2-67961] - Samples were spiked with high concentrations of Rat Ferritin Heavy Chain and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Ferritin Heavy Chain ELISA Kit (Colorimetric) [NBP2-67961] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 6.25 - 400 ng/mL
• Sensitivity: 3.75 ng/mL

Rat Ferritin Heavy Chain ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat FTH in samples. No significant cross-reactivity or interference between Rat FTH and analogues was observed.
• Standard Curve Range: 6.25 - 400 ng/mL
• Sensitivity: 3.75 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.15%
• Intra-Assay: CV% < 4.78%
• Spike Recovery: 87-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FTH1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Ferritin Heavy Chain ELISA Kit (Colorimetric)

• apoferritin
• Cell proliferation-inducing gene 15 protein
• Ferritin H subunit
• ferritin heavy chain
• ferritin, heavy polypeptide 1
• FHC
• FTHEC 1.16.3.1
• FTHL6MGC104426
• PIG15
• placenta immunoregulatory factor
• PLIF
• proliferation-inducing protein 15

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Ferritin Heavy Chain ELISA Kit (NBP2-67961). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FTH1