Mouse Ferritin ELISA Kit (Colorimetric) Summary
| Description |
Ferritin (Mouse) ELISA Kit is a highly sensitive two-site enzyme-linked immunoassay for the quantitative determination of Ferritin in mouse biological samples. |
| Standard Curve Range |
12.5 to 400 ng/mL |
| Sensitivity |
4.709 |
| Assay Type |
Two-site enzyme-linked immunoassay |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
FTH1 |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Anti-Mouse Ferritin ELISA Micro Plate: Twelve removable eight (8) well micro well strips inwell holder frame. Each well is coated with affinity purified anti- Mouse Ferritin.
- Chromogen-Substrate Solution: One vial containing 3,3’,5,5’-tetramethybenzidine (TMB)and hydrogen peroxide in citric acid buffer at pH 3.3.
- Diluent Concentrate (Running Buffer): One bottle containing 5X concentrated diluentrunning buffer.
- Enzyme-Antibody Conjugate 100X: One vial containing affinity purified anti-Mouse Ferritinantibody conjugated with horseradish peroxidase in a stabilizing buffer.
- Mouse Ferritin Calibrator: One vial containing a lyophilized Mouse Ferritin calibrator.
- Stop Solution: One vial containing 0.3 M sulfuric acid. WARNING: Avoid contact with skin
- Wash Solution Concentrate: One bottle containing a 20X concentrated wash solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse Ferritin ELISA Kit (Colorimetric)
Background
This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Ferritin ELISA Kit (KA1941). (Showing 1 - 1 of 1 FAQs).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.