FBXO44 Antibody

Images

 
Western Blot: FBXO44 Antibody [NBP1-84728] - Analysis in human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: FBXO44 Antibody [NBP1-84728] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human brain shows strong nuclear and cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

FBXO44 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SLHRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLSRDQRKEFPNDQVRSQARLRVQVPAVRSAPVVRARASGDLPARPGDHPAEERCQVEGGLPHILQLP
Specificity
Specificity of human FBXO44 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBXO44
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
WB reported in scientific literature (PMID: 25970626). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FBXO44 Recombinant Protein Antigen (NBP1-84728PEP)
Publications
Read Publications using
NBP1-84728 in the following applications:

  • WB
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%). Reactivity reported in scientific literature (PMID: 23086937)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FBXO44 Antibody

  • FBG3 DKFZp781J0852
  • F-box gene 3
  • F-box only protein 44
  • F-box protein 44
  • F-box protein FBX30
  • F-box/G-domain protein 3
  • FBX30FBX6AFbx44
  • FBX44
  • Fbxo6a
  • MGC14140

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FBXO44 Antibody (NBP1-84728)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FBXO44