FBXO28 Antibody - Azide and BSA Free

Western Blot: FBXO28 Antibody [H00023219-B01P] - Analysis of FBXO28 expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: FBXO28 Antibody [H00023219-B01P] - Analysis of purified antibody to FBXO28 on HeLa cell. (antibody concentration 20 ug/ml)

Western Blot: FBXO28 Antibody [H00023219-B01P] - Analysis of FBXO28 expression in HeLa.

Western Blot: FBXO28 Antibody [H00023219-B01P] - Analysis of FBXO28 expression in PC-12.

Western Blot: FBXO28 Antibody [H00023219-B01P] - Analysis of FBXO28 expression in transfected 293T cell line by FBXO28 polyclonal antibody. Lane 1: FBXO28 transfected lysate(40.48 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FBXO28 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse FBXO28 Antibody - Azide and BSA Free (H00023219-B01P) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-FBXO28 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: FBXO28 (NP_055991.1, 1 a.a. - 368 a.a.) full-length human protein. MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKKATEAIDSLRKSKRLRNRK
• Specificity: FBXO28 - F-box protein 28,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: FBXO28
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate for western blot. It has also been used for ELISA. Has also been used for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FBXO28 Antibody - Azide and BSA Free

• F-box only protein 28
• F-box protein 28
• Fbx28
• KIAA0483FLJ10766

Background

Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXO28 Antibody (H00023219-B01P)(1)

Publication using H00023219-B01P

• Cai L, Liu L, Li L et al. SCFFBXO28-mediated self-ubiquitination of FBXO28 promotes its degradation. Cell Signal. 2019-10-31 [PMID: 31678254]

Bioinformatics

• Gene Symbol: FBXO28
• Entrez:
  • 23219(Human)
• Uniprot:
  • NP_055991.1(Human)