FBXL2 Antibody

Immunocytochemistry/Immunofluorescence: FBXL2 Antibody [NB100-1289] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289] - Staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Antibody at 8 ug/mL.

• Reactivity: Hu, Rt, Bv, Ca
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FBXL2 Antibody Summary

• Immunogen: Peptide with sequence VFSNNDEGLINKK-C corresponding to N-Terminus according to NP_036289.2.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FBXL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 8 ug/mL
  • Immunohistochemistry-Paraffin 8 ug/mL
  • Peptide ELISA Detection limit 1:128000
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FBXL2 Antibody

• FBL2FBL3F-box protein containing leucine-rich repeats
• F-box and leucine-rich repeat protein 2DKFZp564P0622
• F-box protein FBL2/FBL3
• F-box/LRR-repeat protein 2

Background

FBXL2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXL2 Antibody (NB100-1289)(1)

Publication using NB100-1289

• Ilyin GP, Rialland M, Pigeon C, Guguen-Guillouzo C. cDNA cloning and expression analysis of new members of the mammalian F-box protein family. Genomics 2000-07-01 [PMID: 10945468]

Bioinformatics

• Gene Symbol: FBXL2
• Entrez:
  • 25827(Human)
• Uniprot:
  • NP_036289.(Human)