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FAT4 Antibody

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Western Blot: FAT4 Antibody [NBP2-78795] - Samples: Whole cell lysate (50 ug) from HeLa and KM12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAT4 antibody NBP2-78795 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.
Western Blot: FAT4 Antibody [NBP2-78795] - Samples: Whole cell lysate (50 ug) from HeLa and KM12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAT4 antibody NBP2-78795 used for WB at ...read more
Immunoprecipitation: FAT4 Antibody [NBP2-78795] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FAT4 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FAT4 Antibody Summary

Immunogen
between 4931 and 4981
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FAT4
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunoprecipitation 8-15 ul/mg

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
TBS containing 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for FAT4 Antibody

  • cadherin family member 14
  • cadherin-related family member 11
  • CDHR11
  • FAT tumor suppressor homolog 4 (Drosophila)
  • FAT-J
  • fat-like cadherin protein FAT-J
  • FLJ77878
  • hFat4
  • NBLA00548
  • putative protein product of Nbla00548

Background

FAT4 (FAT tumor suppressor homolog 4, also know as Fat-J, mFat4, and CDHF14) is a member of the Cadherin superfamily. Members of the Cadherin superfamily positively regulate planar cell polarity (PCP) in drosophila wings and play an important role in cell to cell adhesion interactions. FAT4 and other Cadherin family members have been used in systems medicine; especially in the fields of oncology and neurology (PMID: 16865240). FAT4 is widely expressed in most tissues, especially fetal and infant brain. It is also expressed in brain tumors and colorectal cancer. Furthermore, human breast tumor cell lines and primary tumors show a loss of FAT4 expression. Promoter methylation of a FAT4 allele can inactivate the expression of the FAT4 gene in deleted regions of the Hippo signaling pathway, the pathway that controls organ size (PMID: 19048595).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: FAT1 Antibody [NBP1-84565] - Staining of human rectum shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: FAT1 Antibody [NBP1-84565] - Staining of human prostate shows moderate membranous and cytoplasmic positivity in smooth muscle cells and glandular cells.
Immunohistochemistry-Paraffin: FAT1 Antibody [NBP1-84565] - Staining of human skeletal muscle shows very weak positivity in myocytes.
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Western blot shows lysates of LNCaP human prostate cancer cell line, JAR human choriocarcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PGPEP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6278) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PGPEP‑1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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(5 Publications)
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Applications: WB, IP, ICC
Lysosomal Pro-X
Carboxypeptidase/PRCP ...
AF7164
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Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).
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Immunohistochemistry-Paraffin: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Staining of retina shows strong cytoplasmic positivity in photoreceptors.
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(SZ22-01)
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MEK2 Antibody
(SD207-08)
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Western Blot: MEK2 Antibody (SD207-08) [NBP2-67791] - Analysis of MEK2 on different lysates using anti-MEK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: 293T
Immunocytochemistry/Immunofluorescence: MEK2 Antibody (SD207-08) [NBP2-67791] - Staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MEK2 Antibody (SD207-08) [NBP2-67791] - Analysis of paraffin-embedded mouse lung tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
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Dehydrodolichyl
Diphosphate Synthase ...
NBP1-84975
Immunohistochemistry-Paraffin: Dehydrodolichyl Diphosphate Synthase Antibody [NBP1-84975] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
(1 Publication)
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ERK2 Antibody
(191801) [Unconjugate
MAB1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line,PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ERK2 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for ERK2 at approximately 43 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Species: Hu, Mu, Rt
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PALS1/MPP5 Antibody
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Immunocytochemistry/Immunofluorescence: PALS1/MPP5 Antibody [NB300-952] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: PALS1/MPP5 Antibody [NB300-952] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: PALS1/MPP5 Antibody [NB300-952] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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DHPS Antibody
NBP1-82648
Western Blot: DHPS Antibody [NBP1-82648] - Analysis in control (vector only transfected HEK293T lysate) and DHPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: DHPS Antibody [NBP1-82648] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
(1 Publication)
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LATS2 Antibody
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Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of Mouse brain, using LATS2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: LATS2 Antibody [NBP3-03913] - Rat heart using LATS2 Rabbit pAb at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: LATS2 Antibody [NBP3-03913] - Mouse heart using LATS2 Rabbit pAb at dilution of 1:100 (40x lens).
Species: Hu, Mu, Rt
Applications: WB, IHC
FAT4 Antibody
NBP2-78795
FAT4 Antibody
Species: Hu, Mu
Applications: WB, IP

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Bioinformatics

Gene Symbol FAT4
Uniprot