FAT1 Antibody (FAT-1 3D7/1) - BSA Free

Product Discontinued

FAT1 Antibody (FAT-1 3D7/1) - BSA Free Summary

• Immunogen: Cytoplasmic domain of drosophila Fat
• Localization: Cell Membrane
• Predicted Species: Chimpanzee (92%). Backed by our 100% Guarantee.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: FAT1
• Purity: Thiophilic Chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: 3-8% gradient gels and wet transfer techniques are recommended for the visualisation of FAT1. Positive control(s): Wild type imaginal discs from third instar Drosophila larvae.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Thiophilic Chromatography

Alternate Names for FAT1 Antibody (FAT-1 3D7/1) - BSA Free

• FAT tumor suppressor 1
• FAT tumor suppressor homolog 1 (Drosophila)

Background

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for FAT1 Antibody (NB100-2693) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human and Rat.

reviewed by: Verified Customer WB Human and Rat 03/03/2017

Summary

• Application: Western Blot
• Sample Tested: macrophage
• Species: Human and Rat
• Lot: 596/05

Comments

• Comments: 3-8 PERCENT TRIS ACETATE GEL
  TRANSFER 10V OVERNIGHT
  ANTIBODY 1:500
  SHOWS HIGHER THAN 500 KDA BAND IN RAT, NOT IN HUMAN SAMPLES.

Bioinformatics

• Gene Symbol: FAT1
• Entrez:
  • 2195(Human)