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FAT1 Antibody (FAT-1 3D7/1)

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Product Details

Summary
Reactivity DrSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
FAT-1 3D7/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-2693AF405
Alexa Fluor 488
NB100-2693AF488
Alexa Fluor 647
NB100-2693AF647
Alexa Fluor 700
NB100-2693AF700
Biotin
NB100-2693B
DyLight 350
NB100-2693UV
DyLight 405
NB100-2693V
DyLight 488
NB100-2693G
DyLight 550
NB100-2693R
DyLight 650
NB100-2693C
DyLight 680
NB100-2693FR
DyLight 755
NB100-2693IR
FITC
NB100-2693F
HRP
NB100-2693H
Janelia Fluor 549
NB100-2693JF549
Janelia Fluor 646
NB100-2693JF646
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FAT1 Antibody (FAT-1 3D7/1) Summary

Immunogen
Cytoplasmic domain of drosophila Fat
Localization
Cell Membrane
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
FAT1
Purity
IgM purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
Application Notes
3-8% gradient gels and wet transfer techniques are recommended for the visualisation of Fat-1.
Reviewed Applications
Read 1 Review rated 4
using
NB100-2693 in the following applications:

Reactivity Notes

Expected to cross-react with Rat (85% identity with immunogen) and Chimpanzee (92% identity with immunogen) due to sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Notes

Positive control(s): Wild type imaginal discs from third instar Drosophila larvae..

Alternate Names for FAT1 Antibody (FAT-1 3D7/1)

  • FAT tumor suppressor 1
  • FAT tumor suppressor homolog 1 (Drosophila)

Background

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00376497-D01P
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BMP-1/PCP Antibody
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AF1927
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FAT1 Antibody
(FAT-1 3D7/1)
NB100-2693
FAT1 Antibody (FAT-1 3D7/1)
Species: Dr
Applications: WB, ELISA, ICC/IF, IHC

Publications for FAT1 Antibody (NB100-2693) (0)

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Review for FAT1 Antibody (NB100-2693) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat and Human.

Reviews using NB100-2693:
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Images Ratings Applications Species Date Details
Western Blot FAT1 NB100-2693
Enlarge
reviewed by:
Anonymous
 WB Rat and Human 03/03/2017
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Summary

ApplicationWestern Blot
Sample Testedmacrophage
SpeciesRat and Human
Lot596/05

Comments

Comments3-8 PERCENT TRIS ACETATE GEL
TRANSFER 10V OVERNIGHT
ANTIBODY 1:500
SHOWS HIGHER THAN 500 KDA BAND IN RAT, NOT IN HUMAN SAMPLES.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2693AF405
Alexa Fluor 488 Labeled NB100-2693AF488
Alexa Fluor 647 Labeled NB100-2693AF647
Alexa Fluor 700 Labeled NB100-2693AF700
DyLight 350 Labeled NB100-2693UV
DyLight 405 Labeled NB100-2693V
DyLight 488 Labeled NB100-2693G
DyLight 680 Labeled NB100-2693FR
DyLight 755 Labeled NB100-2693IR
Learn about Custom Antibody Labeling

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Western Blot FAT1 NB100-2693
Enlarge
 Anonymous
03/03/2017
Application: WB
Species: Rat and Human
read this review

Bioinformatics

Gene Symbol FAT1
Entrez