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FAT1 Antibody (FAT-1 3D7/1)

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Product Details

Summary
Reactivity Dr, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
FAT-1 3D7/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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FAT1 Antibody (FAT-1 3D7/1) Summary

Immunogen
Cytoplasmic domain of drosophila Fat
Localization
Cell Membrane
Predicted Species
Chimpanzee (92%). Backed by our 100% Guarantee.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
FAT1
Purity
IgM purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
3-8% gradient gels and wet transfer techniques are recommended for the visualisation of Fat-1.
Reviewed Applications
Read 1 Review rated 4
using
NB100-2693 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified

Notes

Positive control(s): Wild type imaginal discs from third instar Drosophila larvae..

Alternate Names for FAT1 Antibody (FAT-1 3D7/1)

  • FAT tumor suppressor 1
  • FAT tumor suppressor homolog 1 (Drosophila)

Background

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GFER/ALR was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFER/ALR at approximately 20-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Publication)
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Applications: IHC, WB
Dehydrodolichyl
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Immunohistochemistry-Paraffin: Dehydrodolichyl Diphosphate Synthase Antibody [NBP1-87964] - Staining of human Epididymis shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Dehydrodolichyl Diphosphate Synthase Antibody [NBP1-87964] - Staining of human Kidney shows strong membranous and cytoplasmic positivity in cells in tubules and glomeruli.
Immunohistochemistry-Paraffin: Dehydrodolichyl Diphosphate Synthase Antibody [NBP1-87964] - Staining of human Rectum shows strong membranous and cytoplasmic positivity in glandular cells.
(1 Publication)
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Applications: IHC, IHC-P
Peripherin 2/PRPH2
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Western Blot: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Analysis in control (vector only transfected HEK293T lysate) and PRPH2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Staining of human endometrium shows no positivity in glandular cells as expected.
Species: Hu
Applications: IHC, IHC-P, WB
DHPS Antibody
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Immunocytochemistry/Immunofluorescence: DHPS Antibody [NBP1-82648] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.
Western Blot: DHPS Antibody [NBP1-82648] - Analysis in control (vector only transfected HEK293T lysate) and DHPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
(1 Publication)
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Applications: ICC/IF, IHC, IHC-P, WB
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Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).
Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P
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Applications: CyTOF-ready, ICC, ICFlow
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Lysosomal Pro‑X Carboxypeptidase/PRCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7164) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysosomal Pro‑X Carboxypeptidase/PRCP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
FAT4 Antibody -
BSA Free
NBP1-78381
Knockout Validated: FAT4 Antibody - BSA Free [NBP1-78381] - FAT4 Antibody [NBP1-78381] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and BGC-823-FAT4-KO derived xenograft tumors with antibody against FAT4 (NBP1-78381F). Scale bar, 100 um. Image collected and cropped by Citeab from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY licence.
Knockout Validated: FAT4 Antibody [NBP1-78381] - IHC staining of metastatic lung nodules in the nude mice model. Representative images show IHC staining with anti-FAT4 antibody of lung sections from BGC-823-FAT4-KO and BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 i1/4m. Image collected and cropped by Citeab from the following publication (Low FAT4 expression is associated with a poor prognosis in gastric cancer patients. Oncotarget (2018) licensed under a CC-BY licence.
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(6 Publications)
Species: Hu, Mu
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FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(2 Reviews)
(21 Publications)
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Immunohistochemistry-Paraffin: FATP2/SLC27A2 Antibody [NBP3-05516] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: FATP2/SLC27A2 Antibody [NBP3-05516] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: FATP2/SLC27A2 Antibody [NBP3-05516] - Analysis in human kidney and pancreas tissues using Anti-SLC27A2 antibody. Corresponding SLC27A2 RNA-seq data are presented for the same tissues.
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Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of Jurkat cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP.
(1 Publication)
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Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human YAP1 Polyclonal Antibody (Catalog # NB110-58358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 50 ug/mL of Rabbit Anti-YAP1 Polyclonal Antibody (Catalog # NB110-58358). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at 1:200 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear and cytoplasm in trophoblast cells.
(2 Reviews)
(60 Publications)
Species: Ca, Hu, Mu, Rt, Ze
Applications: ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB

Publications for FAT1 Antibody (NB100-2693) (0)

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Review for FAT1 Antibody (NB100-2693) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.

Reviews using NB100-2693:
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Western Blot FAT1 NB100-2693
Enlarge 		4
reviewed by:
AMIT LAHIRI
 WB Human and Rat 03/03/2017
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Summary

ApplicationWestern Blot
Sample Testedmacrophage
SpeciesHuman and Rat
Lot596/05

Comments

Comments3-8 PERCENT TRIS ACETATE GEL
TRANSFER 10V OVERNIGHT
ANTIBODY 1:500
SHOWS HIGHER THAN 500 KDA BAND IN RAT, NOT IN HUMAN SAMPLES.

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Western Blot FAT1 NB100-2693
Enlarge
 AMIT LAHIRI
03/03/2017
Application: WB
Species: Human and Rat
read this review

Bioinformatics

Gene Symbol FAT1
Entrez