FAT1 Antibody (FAT-1 3D7/1)

Images

 
There are currently no images for FAT1 Antibody (NB100-2693).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity DrSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
FAT-1 3D7/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FAT1 Antibody (FAT-1 3D7/1) Summary

Immunogen
Cytoplasmic domain of drosophila Fat
Localization
Cell Membrane
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
FAT1
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
Application Notes
3-8% gradient gels and wet transfer techniques are recommended for the visualisation of Fat-1.
Reviewed Applications
Read 1 Review rated 4
using
NB100-2693 in the following applications:

Reactivity Notes

Expected to cross-react with Rat (85% identity with immunogen) and Chimpanzee (92% identity with immunogen) due to sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Notes

Positive control(s): Wild type imaginal discs from third instar Drosophila larvae..

Alternate Names for FAT1 Antibody (FAT-1 3D7/1)

  • FAT tumor suppressor 1
  • FAT tumor suppressor homolog 1 (Drosophila)

Background

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB7940
Species: Hu
Applications: WB, IHC
NBP1-84975
Species: Hu
Applications: IHC, IHC-P
NBP1-86687
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-82648
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-53235
Species: Hu
Applications: Flow, ICC/IF, IHC-P, PAGE
H00376497-D01P
Species: Hu, Mu
Applications: WB, ELISA
AF7164
Species: Hu
Applications: WB
NBP1-78381
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF6278
Species: Hu, Mu
Applications: WB
AF1927
Species: Hu
Applications: WB, IP, ICC
NBP1-87914
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF1443
Species: Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP2-37738
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-90642
Species: Hu
Applications: IHC, IHC-P
NBP1-85792
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF3159
Species: Mu
Applications: WB, IHC
NB110-58358
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-2693
Species: Dr
Applications: WB, ELISA, ICC/IF, IHC

Publications for FAT1 Antibody (NB100-2693) (0)

There are no publications for FAT1 Antibody (NB100-2693).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for FAT1 Antibody (NB100-2693) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat and Human.

Reviews using NB100-2693:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat and Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FAT1 NB100-2693
Enlarge
reviewed by:
Anonymous
WB Rat and Human 03/03/2017
View

Summary

ApplicationWestern Blot
Sample Testedmacrophage
SpeciesRat and Human
Lot596/05

Comments

Comments3-8 PERCENT TRIS ACETATE GEL
TRANSFER 10V OVERNIGHT
ANTIBODY 1:500
SHOWS HIGHER THAN 500 KDA BAND IN RAT, NOT IN HUMAN SAMPLES.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FAT1 Antibody (NB100-2693) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2693AF405
Alexa Fluor 488 Labeled NB100-2693AF488
Alexa Fluor 647 Labeled NB100-2693AF647
Alexa Fluor 700 Labeled NB100-2693AF700
DyLight 350 Labeled NB100-2693UV
DyLight 405 Labeled NB100-2693V
DyLight 488 Labeled NB100-2693G
DyLight 680 Labeled NB100-2693FR
DyLight 755 Labeled NB100-2693IR

Additional FAT1 Products

Bioinformatics Tool for FAT1 Antibody (NB100-2693)

Discover related pathways, diseases and genes to FAT1 Antibody (NB100-2693). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FAT1 Antibody (NB100-2693)

Discover more about diseases related to FAT1 Antibody (NB100-2693).
 

Pathways for FAT1 Antibody (NB100-2693)

View related products by pathway.

PTMs for FAT1 Antibody (NB100-2693)

Learn more about PTMs related to FAT1 Antibody (NB100-2693).
 

Research Areas for FAT1 Antibody (NB100-2693)

Find related products by research area.

Blogs on FAT1

There are no specific blogs for FAT1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
03/03/2017
Application: WB
Species: Rat and Human

Bioinformatics

Gene Symbol FAT1
Entrez