Fascin 2 Antibody

Immunohistochemistry-Paraffin: Fascin 2 Antibody [NBP1-06036] - Staining of paraffin embedded Mouse Brain. Antibody at 4 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Mu, Hu, Rt, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Fascin 2 Antibody Summary

• Description: Novus Biologicals Goat Fascin 2 Antibody (NBP1-06036) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Fascin 2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CHHRGSNQLDTNR corresponding to internal region according to NP_036550.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1).
• Predicted Species: Human (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FSCN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4 ug/mL
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave bands at approx. 75 kDa band and 22 kDa band in mouse eye lysates after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 57.4 kDa band according to NP_001070650.1 and 55.1 kDa band according to NP_036550.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P successful usage on sections of mouse cochlea is reported in scientific literature (PMID: 20660251).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Fascin 2 Antibody

• fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotuspurpuratus)
• fascin-2
• Retinal fascin
• retinal)
• RFSNRP30fascin (Strongylocentrotus purpuratus) homolog 2 (actin-bundling protein

Background

The Fascin 2 gene encodes a member of the fascin protein family. Fascins crosslink actin into filamentous bundles within dynamic cell extensions. This family member is proposed to play a role in photoreceptor disk morphogenesis. A mutation in this gene results in

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fascin 2 Antibody (NBP1-06036)(2)

Publications using NBP1-06036

• Shin JB, Longo-Guess CM, Gagnon LH et al. The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice. J Neurosci 2010-07-21 [PMID: 20660251] (IHC-P, Mouse)
• Vignjevic D, Kojima S, Aratyn Y et al. Role of fascin in filopodial protrusion. J Biol 2006-09-11 [PMID: 16966425]

Bioinformatics

• Gene Symbol: FSCN2
• Entrez:
  • 25794(Human)
  • 238021(Mouse)
  • 303741(Rat)
• Uniprot:
  • NP_001070650.1(Human)