FARP1 Antibody

Flow Cytometry: FARP1 Antibody - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence: FARP1 Antibody - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence: FARP1 Antibody [NBP2-76803] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FARP1 Antibody Summary

• Immunogen: Peptide with sequence GEIEQRPTPGSRL-C, from the N Terminus of the protein sequence according to NP_005757.1; NP_001001715.2; NP_001273768.1.
• Specificity: This antibody is expected to recognize isoform 1 (NP_005757.1), isoform 2 (NP_001001715.2), and isoform 3 (NP_001273768.1)
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FARP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Peptide ELISA Detection Limit 1:32000
• Application Notes: WB: Preliminary experiments in human liver, kidney and lung lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FARP1 Antibody

• CDEPPleckstrin homology domain-containing family C member 2
• Chondrocyte-derived ezrin-like protein
• FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
• FERM, RhoGEF and pleckstrin domain-containing protein 1
• MGC87400
• PLEKHC2PH domain-containing family C member 2

Background

This gene was originally isolated through subtractive hybridization due to its increased expression in differentiated chondrocytes versus dedifferentiated chondrocytes. The resulting protein contains a predicted ezrin-like domain, a Dbl homology domain, and a pleckstrin homology domain. It is believed to be a member of the band 4.1 superfamily whose members link the cytoskeleton to the cell membrane. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FARP1
• Entrez:
  • 10160(Human)
• Uniprot:
  • NP_001001715.2(Human)
  • NP_001273768.1(Human)
  • NP_005757(Human)