FAM3A Antibody (7-B9)

Western Blot: FAM3A Antibody (7-B9) [NBP2-75487] - Western blot analysis of FAM3A on recombinant protein using anti-FAM3A antibody at 1/1000 dilution.

Immunohistochemistry-Paraffin: FAM3A Antibody (7-B9) [NBP2-75487] - Analysis of paraffin-embedded mouse heart tissue using anti-FAM3A antibody. Counter stained with hematoxylin.

Flow Cytometry: FAM3A Antibody (7-B9) [NBP2-75487] - Analysis of A431 cells with FAM3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: FAM3A Antibody (7-B9) [NBP2-75487] - Analysis of paraffin-embedded human kidney tissue using anti-FAM3A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FAM3A Antibody (7-B9) [NBP2-75487] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-FAM3A antibody. Counter stained with hematoxylin.

Product Discontinued

FAM3A Antibody (7-B9) Summary

• Immunogen: Recombinant protein within Human FAM3A 1-230 / 230. (SwissProt: P98173 Human; SwissProt: Q9D8T0 Mouse)
• Localization: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FAM3A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:2000-1:5000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for FAM3A Antibody (7-B9)

• 2.19
• Cytokine-like protein 2-19
• DLD
• DXS560S
• FAM3A
• family with sequence similarity 3, member A
• member A protein
• XAP-7

Background

In similar amounts in testis, pancreas, adrenal, placenta, brain, fetal brain, liver, kidney,skeletal muscle and heart

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FAM3A Antibody (NBP2-75487)(1)

Publication using NBP2-75487

• Yan S, Jiang C, Li H et al. FAM3A protects chondrocytes against interleukin-1 beta-induced apoptosis through regulating PI3K/Akt/mTOR pathway Biochem Biophys Res Commun 2019-06-19 [PMID: 31208715]

Bioinformatics

• Gene Symbol: FAM3A