FAK [p Tyr861] Antibody

Western Blot: FAK [p Tyr861] Antibody [NBP2-68146] - Extracts of 3T3-L1 cells unstimulated or stimulated with 50 ng/ml LIF for 15 minutes (2-4) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4C, then incubated with JAK2 (pYpY1007/1008) antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the phosphopeptide immunogen (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F (ab')2 anti-rabbit IgG HRP-conjugate and signals were detected using the Pierce SuperSignal method. The data show that only the phosphopeptide corresponding to JAK2 (pYpY1007/1008) blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the up-regulation of JAK2 (pYpY1007/1008) with LIF treatment in this cell system.

Immunocytochemistry/Immunofluorescence: FAK [p Tyr861] Antibody [NBP2-68146] - Immunofluorescence analysis of FAK [p Tyr861] Antibody was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with FAK [p Tyr861] Antibody Rabbit Polyclonal Antibody at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody conjugated to Alexa Fluor 488 at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with a liquid mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 555 Rhodamine Phalloidin. Panel d is a merged image showing Cell Junctional localization. Panel e is a no primary antibody control. The images were captured at 60x magnification.

Product Discontinued

FAK [p Tyr861] Antibody Summary

• Immunogen: Chemically synthesized phosphopeptide obtained from the region of human FAK that contains Tyr861. The sequence is conserved in mouse, rat, chicken and frog.
• Modification: p Tyr861
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:1000

Reactivity Notes

Amphibian, Canine, Hamster, Non-human primate, Rat, Xenopus, Zebrafish

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Dulbecco's PBS, pH 7.3, with 50% glyceroll and 1mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Alternate Names for FAK [p Tyr861] Antibody

• EC 2.7.10
• FADK 1
• FADK1
• FADKFAK-related non-kinase polypeptide
• FAK
• FAK1FRNK
• FAKEC 2.7.10.2
• focal adhesion kinase 1
• pp125FAK
• Protein-tyrosine kinase 2
• PTK2 protein tyrosine kinase 2
• PTK2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTK2