FAK Antibody (2C3)

Western Blot: FAK Antibody (2C3) [H00005747-M01] - PTK2 monoclonal antibody (M01), clone 2C3 Analysis of PTK2 expression in Hela S3 NE.

Proximity Ligation Assay: FAK Antibody (2C3) [H00005747-M01] - Analysis of protein-protein interactions between STAT1 and PTK2. HeLa cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-PTK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Immunoprecipitation: FAK Antibody (2C3) [H00005747-M01] - Analysis of PTK2 transfected lysate using anti-PTK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTK2 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: FAK Antibody (2C3) [H00005747-M01] - Detection limit for recombinant GST tagged PTK2 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

FAK Antibody (2C3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PTK2 (AAH28733, 355 a.a. ~ 490 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQ
• Marker: Focal Adhesion Marker
• Specificity: PTK2 - PTK2 protein tyrosine kinase 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FAK Antibody (2C3)

• EC 2.7.10
• FADK 1
• FADK1
• FADKFAK-related non-kinase polypeptide
• FAK
• FAK1FRNK
• FAKEC 2.7.10.2
• focal adhesion kinase 1
• pp125FAK
• Protein-tyrosine kinase 2
• PTK2 protein tyrosine kinase 2
• PTK2

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FAK Antibody (H00005747-M01)(3)

Publications using H00005747-M01

• Shibayama Y, Kubo Y, Nakagawa T, Iseki K MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1A Biol. Pharm. Bull. 2019-08-02 [PMID: 31366868] (WB, Human)
• Li JP, Fu YN, Chen YR, Tan TH. JNK Pathway-associated Phosphatase Dephosphorylates Focal Adhesion Kinase and Suppresses Cell Migration. J Biol Chem. 2010-02-19 [PMID: 20018849]
• Li JP, Fu YN, Chen YR, Tan TH. JNK pathway-associated phosphatase dephosphorylates focal adhesion kinase and suppresses cell migration. J Biol Chem 285(8):5472-8. 2010-02-19 [PMID: 2001884]

Bioinformatics

• Gene Symbol: PTK2
• Entrez:
  • 5747(Human)
• OMIM:
  • 600758(Human)
• Uniprot:
  • AAH28733(Human)