FAK Antibody (10H7E9) - BSA Free

Western Blot: FAK Antibody (10H7E9) [NBP1-47494] - Analysis using FAK mAb against FAK(AA: 354-533)-hIgGFc transfected HEK293 cell lysate.

Immunocytochemistry/Immunofluorescence: FAK Antibody (10H7E9) [NBP1-47494] - Analysis of A549 cells using FAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: FAK Antibody (10H7E9) [NBP1-47494] - Analysis of Raji cells using FAK mouse mAb (green) and negative control (purple).

Product Discontinued

FAK Antibody (10H7E9) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human FAK expressed in E. Coli.
• Marker: Focal Adhesion Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTK2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 119 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for FAK Antibody (10H7E9) - BSA Free

• EC 2.7.10
• FADK 1
• FADK1
• FADKFAK-related non-kinase polypeptide
• FAK
• FAK1FRNK
• FAKEC 2.7.10.2
• focal adhesion kinase 1
• pp125FAK
• Protein-tyrosine kinase 2
• PTK2 protein tyrosine kinase 2
• PTK2

Background

Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTK2
• Entrez:
  • 5747(Human)
• Uniprot:
  • Q05397(Human)