Factor VIII Antibody (RFF-VIIIC/10)

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Western Blot: FACTOR VIII Antibody (RFF-VIIIC/10) [NBP1-41021] - Recombinant Factor VIII probed with Mouse anti Human Factor VIII

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, RIA
Clone
RFF-VIIIC/10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Factor VIII Antibody (RFF-VIIIC/10) Summary

Immunogen
Affinity purified human Factor VIII
Specificity
This antibody recognises human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Clone RFF-VIIIC/10 recognises an epitope within the non-functional, middle domain of full-length Factor VIII.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
F8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • ELISA 1:100-1:2000
  • Radioimmunoassay 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Factor VIII Antibody (RFF-VIIIC/10)

  • AHF
  • Antihemophilic factor
  • coagulation factor VIII, procoagulant component
  • coagulation factor VIIIc
  • DXS1253E
  • F8Ccoagulation factor VIII
  • factor VIII F8B
  • FVIII
  • HEMAF8B
  • Procoagulant component

Background

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on Factor VIII.

Factor VIII - a key factor in the clotting process
Hemostasis, or blood clotting, follows tissue injury and involves the deployment of essential plasma procoagulants (such as prothrombin, and Factors X, IX, V, and VIII) that trigger the blood coagulation cascade. This cascade leads to the formation...  Read full blog post.

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Bioinformatics

Gene Symbol F8
Entrez
Uniprot