Factor VIII Antibody (RFF-VIIIC/10) - BSA Free

Product Discontinued

Factor VIII Antibody (RFF-VIIIC/10) - BSA Free Summary

• Immunogen: Affinity purified human Factor VIII
• Specificity: This antibody recognises human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Clone RFF-VIIIC/10 recognises an epitope within the non-functional, middle domain of full-length Factor VIII.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: F8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Radioimmunoassay 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Factor VIII Antibody (RFF-VIIIC/10) - BSA Free

• AHF
• Antihemophilic factor
• coagulation factor VIII, procoagulant component
• coagulation factor VIIIc
• DXS1253E
• F8Ccoagulation factor VIII
• factor VIII F8B
• FVIII
• HEMAF8B
• Procoagulant component

Background

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F8
• Entrez:
  • 2157(Human)
• Uniprot:
  • P00451(Human)