EYA2 Antibody (2F8) - Azide and BSA Free

Western Blot: EYA2 Antibody (2F8) [H00002139-M04] - Analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8. Lane 1: EYA2 transfected lysatE (59.2 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: EYA2 Antibody (2F8) [H00002139-M04] - Analysis of monoclonal antibody to EYA2 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: EYA2 Antibody (2F8) [H00002139-M04] - Analysis of EYA2 expression in IMR-32 (Cat # L008V1).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EYA2 Antibody (2F8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EYA2 (NP_742108, 164 a.a. ~ 271 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYG
• Specificity: EYA2 (2F8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EYA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EYA2 Antibody (2F8) - Azide and BSA Free

• EAB1EC 3.1.3.48
• eyes absent (Drosophila) homolog 2
• eyes absent homolog 2 (Drosophila)
• eyes absent homolog 2
• MGC10614

Background

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Five transcript variants encoding three distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EYA2 Antibody (H00002139-M04)(1)

Publication using H00002139-M04

• Jia S, Zhixin Q, Qiulian W et al. A FBXO7/EYA2-SCF FBXW7 axis promotes AXL-mediated maintenance of mesenchymal and immune evasion phenotypes of cancer cells. Mol Cell. 2022-02-18 [PMID: 35182481]

Bioinformatics

• Gene Symbol: EYA2
• Entrez:
  • 2139(Human)
• OMIM:
  • 601654(Human)
• Uniprot:
  • NP_742108(Human)