Exonuclease 1 Antibody

Western Blot: Exonuclease 1 Antibody [NB100-93394] - (0.2ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Exonuclease 1 Antibody Summary

• Immunogen: Peptide with sequence C-HRNYSPRPESGT corresponding to internal region according to NP_003677.3, NP_006018.3, NP_569082.1.
• Specificity: This antibody is expected to recognise all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EXO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.2 - 0.5 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1 kDa band according to NP_003677.3). An additional band of unknown identity was also consistently observed at 38 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Exonuclease 1 Antibody

• EXOI
• exonuclease 1
• Exonuclease I
• HEX1rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
• hExo1
• hExoIEC 3.1

Background

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Exonuclease 1 Antibody (NB100-93394)(1)

Publication using NB100-93394

• Kim JC, Roh SA, Yoon YS et al. MLH3 and EXO1 alterations in familial colorectal cancer patients not fulfilling Amsterdam criteria. Cancer Genet Cytogenet 2007-07-15 [PMID: 17656264]

Bioinformatics

• Gene Symbol: EXO1
• Entrez:
  • 9156(Human)
• Uniprot:
  • Q9UQ84(Human)