ETS2 Antibody (PCRP-ETS2-1D9)

Immunocytochemistry/Immunofluorescence: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ETS2 Antibody (PCRP-ETS2-1D9) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877] - Flow cytometric analysis of PFA-fixed HeLa cells. ETS2 antibody (PCRP-ETS2-1D9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ETS2 Antibody (PCRP-ETS2-1D9).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-ETS2-1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ETS2 Antibody (PCRP-ETS2-1D9) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14209)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human ETS2 protein (Uniprot: P15036)
• Localization: Nucleus
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ETS2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for ETS2 Antibody (PCRP-ETS2-1D9)

• ETS2IT1
• human erythroblastosis virus oncogene homolog 210protein C-ets-2
• oncogene ETS-2
• v-ets avian erythroblastosis virus E2 oncogene homolog 2
• v-ets avian erythroblastosis virus E26 oncogene homolog 2
• v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)

Background

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ETS2
• Uniprot:
  • P15036(Human)