Ets-1 Antibody (800710) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: IHC
• Clone: 800710
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Ets-1 Antibody (800710) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Ets-1
  Glu127-Val230
  Accession # P14921
• Specificity: Detects human Ets-1 in ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ets-1 Antibody (800710) [Alexa Fluor® 405]

• Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1
• ets protein
• Ets1
• Ets-1
• EWSR2
• FLJ10768
• p54
• Protein C-Ets-1
• v-ets avian erythroblastosis virus E2 oncogene homolog 1
• v-ets avian erythroblastosis virus E26 oncogene homolog 1
• v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• v-ets

Background

Ets-1 (E26 Transformation-Specific 1; also p54 and c-ets1) is a 52-54 kDa member of the ETS family of proteins. It is found in multiple cell types, and serves as a transcriptional regulator (generally activator) of multiple target genes, including prolactin, the transferrin receptor, and Cyclin E. By upregulating Cyclin E and CDK2 genes, it promotes cell-cycle progression. Ets‑1 forms complexes with both transcriptional activators (AP-1 and GHF-1) and repressors (MafB and Daxx). Human Ets‑1 is 441 amino acids (aa) in length. It contains one PNT domain (aa 51-136) that binds ERK2, and a DNA-binding ETS domain (aa 335-415). There are two SUMOylation sites, plus four utilized phosphorylation and acetylated lysine sites. At least four potential isoform variants are reported. One shows a deletion of aa 244-330 (termed isoform 1B), a second shows a deletion of aa 262-331, a third contains a deletion of aa 28-244, and a fourth possesses an 11 aa substitution for aa 262-441. Over aa 127-230, human Ets‑1 shares 95% aa identity with mouse Ets‑1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P14921(Human)