ERManI Antibody (3C2)

Western Blot: ERManI Antibody (3C2) [NBP2-13167] - Western blot analysis of ERManI expression in 1) NTERA-2 and 2) A-431 whole cell lysates.

Immunocytochemistry/Immunofluorescence: ERManI Antibody (3C2) [NBP2-13167] - ERManI antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: ERManI Antibody (3C2) [NBP2-13167] - IHC staining of ERManI in human breast cancer using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu(-)
• Applications: WB, ELISA, ICC/IF, IHC, IP, KO
• Clone: 3C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ERManI Antibody (3C2) Summary

• Immunogen: Full length human recombinant ERManI [Swiss-Prot# Q9UKM7]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAN1B1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:100
  • Knockout Validated reported in scientific literature (PMID 26205822)
  • Western Blot 1:500-1:1000
• Application Notes: In ICC/IF, endoplasmic reticulum membrane staining was observed in HeLa cells. In Western Blot, a band is seen ~90 kDa representing ERManI. In IHC-P, staining was observed in the endoplasmic reticulum of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  A-431 Whole Cell Lysate
  NTERA-2 Whole Cell Lysate

Reactivity Notes

Does not react with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine (pH 7.5) and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for ERManI Antibody (3C2)

• alpha 1,2-mannosidase
• EC 3.2.1.113
• endoplasmic reticulum alpha-mannosidase 1
• endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1
• endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
• ER alpha 1,2-mannosidase
• ER alpha-1,2-mannosidase
• ER ManI
• ER mannosidase 1
• ERMan1
• MAN1B1
• Man9GlcNAc2-specific processing alpha-mannosidase
• Man9GlcNAc2-specific-processing alpha-mannosidase
• MANA-ER
• Mannosidase alpha class 1B member 1
• mannosidase, alpha, class 1B, member 1
• MRT15
• RP11-350O14.2

Background

ERManI, also known as endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase or MAN1B1, is a putative ER resident mannosidase that plays a rate-limiting role in generating a signal that targets misfolded N-linked glycoproteins for ER-associated degradation (ERAD). ERManI codes for an enzyme that converts Man9GlcNAc to Man8GlcNAc isomer B during N-glycan biosynthesis. It also facilitates the trimming of Man5-6GlcNAc2 in the endoplasmic reticulum associated degradation pathway. Multiple transcript variants of ERManI can result from alternative splicing, and a related pseudogene can be found on chromosome 11. Research has found that mutations on the ERManI gene can result in autosomal-recessive intellectual disabilities and developmental delays (PMID: 21763484). ERManI is predominately localized to the Golgi and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERManI Antibody (NBP2-13167)(3)

Publications using NBP2-13167

• Frabutt DA, Wang B, Riaz S et al. Innate sensing of influenza A virus hemagglutinin glycoproteins by the host endoplasmic reticulum (ER) stress pathway triggers a potent antiviral response via ER-associated protein degradation. J. Virol. 2017-10-18 [PMID: 29046440] (Human)
• Zhou T, Frabutt DA, Moremen KW, Zheng YH et al. ERManI is required for HIV-1 envelope glycoprotein degradation via endoplasmic reticulum-associated protein degradation pathway J. Biol. Chem. 2015-07-23 [PMID: 26205822] (WB, Human)
• Rymen D, Peanne R, Millon MB et al. MAN1B1 Deficiency: An Unexpected CDG-II. PLoS Genet 2013-12-01 [PMID: 24348268] (WB, Human)

Bioinformatics

• Gene Symbol: MAN1B1
• Entrez:
  • 11253(Human)
• Uniprot:
  • Q9UKM7(Human)