Recombinant Human ERK2 Protein

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SDS-Page: Recombinant Human ERK2 Protein [NBP1-30309] - 15% SDS-Page (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human ERK2 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-360 of Human ERK2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
MAPK1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
43.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP1-30309.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human ERK2 Protein

  • EC 2.7.11
  • EC 2.7.11.24
  • ERK
  • ERK2
  • ERK-2
  • ERK2MAP kinase isoform p42
  • ERT1
  • Extracellular signal-regulated kinase 2
  • MAP kinase 1
  • MAP kinase 2
  • MAPK 1
  • MAPK1
  • MAPK2
  • mitogen-activated protein kinase 1
  • Mitogen-activated protein kinase 2
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • p42-MAPK
  • PRKM1
  • PRKM1MAPK 2
  • PRKM2
  • protein tyrosine kinase ERK2

Background

MAPK1, also known as ERK(extracellular signal-regulated kinase), acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Recombinant human MAPK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for ERK2 Recombinant Protein (NBP1-30309)(2)

Reviews for ERK2 Recombinant Protein (NBP1-30309) (0)

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FAQs for ERK2 Recombinant Protein (NBP1-30309). (Showing 1 - 2 of 2 FAQs).

  1. What is the sequence of the His-Tag? Is it N-terminal or C-terminal? I am using the protein in mass spectrometry experiments and knowing the exact sequence is very important.
    • The amino acid sequence of the His-tag is: MGSSHHHHHH SSGLVPRGSH The His-tag is N-terminal.
  2. Do you have any info on the potential activity or 3D structure of product NBP1-30309? Do you expect this protein to be folded into the correct tertiary structure? Any info on tag (6x his) effects for this protein? Do you have any information from a CD spectra or solubility?
    • NBP1-30309 comes in 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT and 10% glycerol. In this reducing buffer, it is not expected to have proper folding and/or activity. This protein is intended to be used as a positive control for Western blots and ELISA.

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Blogs on ERK2.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. Enc...  Read full blog post.

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Bioinformatics

Gene Symbol MAPK1
Entrez