ERK1/ERK2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (798508) [Unconjugated]

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 5 minutes and 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB1825) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-ERK1 (T202/Y204) and Phospho-ERK2 (T185/Y187) at approximately 43 and 41 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 798508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ERK1/ERK2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (798508) [Unconjugated] Summary

• Description: Antibody detects ERK1 [p Thr202, p Tyr204] / ERK2 [p Thr185, p Tyr187]
• Immunogen: Phosphopeptide containing the ERK1 T202/Y204 site
• Modification: p Thr202, p Tyr204, p Thr185, p Tyr187
• Specificity: Detects human ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK1/ERK2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (798508) [Unconjugated]

• ERK1
• ERK-1
• ERK1/ERK2
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAPK 1
• Microtubule-associated protein 2 kinase
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• p44-MAPK
• PRKM3

Background

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1/ERK2 Antibody (MAB1825)(2)

Publications using MAB1825

• M Sisto, L Lorusso, G Ingravallo, D Ribatti, S Lisi TGFbeta1-Smad canonical and -Erk noncanonical pathways participate in interleukin-17-induced epithelial-mesenchymal transition in Sj�gren's syndrome Lab. Invest., 2020-01-10;100(6):824-836. 2020-01-10 [PMID: 31925325] (Flow Cytometry, Western Blot, Human)
• S Álvarez-Te, ST Menéndez, MÁ Villaronga, JP Rodrigo, L Manterola, L de Villala, JC de Vicente, L Alonso-Dur, MP Fernández, CH Lawrie, JM García-Ped Dysregulation of Mir-196b in Head and Neck Cancers Leads to Pleiotropic Effects in the Tumor Cells and Surrounding Stromal Fibroblasts Sci Rep, 2017-12-19;7(1):17785. 2017-12-19 [PMID: 29259267] (Western Blot, Human)