ERCC6L Antibody

Immunohistochemistry-Paraffin: ERCC6L Antibody [NBP2-13969] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: ERCC6L Antibody [NBP2-13969] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: ERCC6L Antibody [NBP2-13969] - Staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ERCC6L Antibody [NBP2-13969] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.

Product Discontinued

ERCC6L Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: IIWIRLVPLQEEIYRKFVSLDHIKELLMETRSPLAELGVLKKLCDHPRLLSARACCLLNLGTFSAQDGNEGEDSPDVDHIDQVTDDTLMEESGKMIFLMDLL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERCC6L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ERCC6L Antibody

• ATP-dependent helicase ERCC6-like
• DNA excision repair protein ERCC-6-like
• EC 3.6.1
• EC 3.6.4.12
• excision repair cross-complementing rodent repair deficiency, complementationgroup 6-like
• excision repair protein ERCC6-like
• FLJ20105
• MGC131695
• PICHexcision repair cross-complementing rodent repair deficiency complementationgroup 6 - like
• PLK1-interacting checkpoint helicase
• SNF2/RAD54 family protein
• Tumor antigen BJ-HCC-15

Background

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERCC6L Antibody (NBP2-13969)(1)

Publication using NBP2-13969

• Stok C, Tsaridou S, van den Tempel N et al. FIRRM/C1orf112 is synthetic lethal with PICH and mediates RAD51 dynamics Cell reports 2023-06-21 [PMID: 37347663] (WB, ICC/IF, Human)
  
  Details:
  Dilution: 1:200; HAP1 Cells

Bioinformatics

• Gene Symbol: ERCC6L