| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Concentration | 0.5 mg/ml |
| Description | A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-273 of Human ERCC1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMDPGKD KEGVPQPSGP PARKKFVIPL DEDEVPPGVA KPLFRSTQSL PTVDTSAQAA PQTYAEYAIS QPLEGAGATC PTGSEPLAGE TPNQALKPGA KSNSIIVSPR QRGNPVLKFV RNVPWEFGDV IPDYVLGQST CALFLSLRYH NLHPDYIHGR LQSLGKNFAL RVLLVQVDVK DPQQALKELA KMCILADCTL ILAWSPEEAG RYLETYKAYE QKPADLLMEK LEQDFVSRSL EQLIAASRED LALCPGLGPQ KARRLFDVLH EPFLKVP |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | ERCC1 |
| Purity | >90%, by SDS-PAGE |
| Dilutions |
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| Theoretical MW | 32.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Purity | >90%, by SDS-PAGE |
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| Gene Symbol | ERCC1 |
![SDS-Page: Recombinant Human ERCC1 Protein [NBP1-98981] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-ERCC1-Protein-SDS-Page-NBP1-98981-img0001.jpg "SDS-Page: Recombinant Human ERCC1 Protein [NBP1-98981] - 15% SDS-PAGE (3ug)")
![Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Anti-ERCC1 immunohistochemistry on whole sections of a lung squamous cell carcinoma, using Mab 8F1 and D-10 with CC1-mono and H2-60 protocols. Note increased cytosolic background with H2-60. Arrow: Necrotic centre. Arrowhead: Stromal axis. 100 A- original magnification. Image collected and cropped by Citeab from the following publication (Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10. J Clin Bioinforma (2011) licensed under a CC-BY license.](https://images.novusbio.com/images/ERCC1-Antibody-8F1-Immunohistochemistry-Paraffin-NB500-704-img0005.jpg "Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Anti-ERCC1 immunohistochemistry on whole sections of a lung squamous cell carcinoma, using Mab 8F1 and D-10 with CC1-mono and H2-60 protocols. Note increased cytosolic background with H2-60. Arrow: Necrotic centre. Arrowhead: Stromal axis. 100 A- original magnification. Image collected and cropped by Citeab from the following publication (Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10. J Clin Bioinforma (2011) licensed under a CC-BY license.")