ErbB3/Her3 [p Tyr1289] Antibody (0004) - Azide and BSA Free

Western Blot: ErbB3/Her3 [p Tyr1289] Antibody (0004) [NBP3-30737] - Western blot analysis of extracts from serum-starved MCF-7, untreated (-) or treated with neuregulin-1 (100 ng/mL, 5 min) using (Cat# NBP3-30737) at 1:2000 dilution (upper), or Rabbit Polyclonal HER3/ERBB3 Antibody at 1:2000 dilution (middle), or Mouse Monoclonal Beta-Tubulin Loading Control Antibody at 1:2000 dilution (lower)

Western Blot: ErbB3/Her3 [p Tyr1289] Antibody (0004) [NBP3-30737] - Western blot analysis of extracts from serum-starved MCF-7, untreated (line A); treated with neuregulin-1 (100ng/ml, 5min; +) (line B); treated with neuregulin-1 and -phosphatase (line C) using NBP3-30737 at 1:100000 dilution.

• Reactivity: Hu, Pm-Cm
• Applications: WB
• Clone: 0004
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ErbB3/Her3 [p Tyr1289] Antibody (0004) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit ErbB3/Her3 [p Tyr1289] Antibody (0004) - Azide and BSA Free (NBP3-30737) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Tyr1289 of the Human ErbB3/Her3
• Modification: p Tyr1289
• Source: HEK293
• Predicted Species: Cynomolgus Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ERBB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for ErbB3/Her3 [p Tyr1289] Antibody (0004) - Azide and BSA Free

• c-erbB3
• EC 2.7.10
• EC 2.7.10.1
• ErbB3
• ErbB-3
• erbB3-S
• HER3
• HER3c-erbB-3
• LCCS2
• lethal congenital contracture syndrome 2
• MDA-BF-1
• MGC88033
• p180-ErbB3
• p45-sErbB3
• p85-sErbB3
• Proto-oncogene-like protein c-ErbB-3
• receptor tyrosine-protein kinase erbB-3
• Tyrosine kinase-type cell surface receptor HER3
• v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERBB3