ER beta/NR3A2 Antibody (PPG5/10) - BSA Free

Immunocytochemistry/Immunofluorescence: ER beta/NR3A2 Antibody (PPG5/10) - Azide and BSA Free [NBP2-61944] - ICC/IF showing estrogen receptor beta (red) in human endocervical epithelial cells. Nuclei are stained with DAPI (blue). 40x magnification. ICC/IF image submitted by a verified customer review.

Product Discontinued

ER beta/NR3A2 Antibody (PPG5/10) - BSA Free Summary

• Immunogen: Synthetic peptide CEDSKSKEGSQNPQSQ derived from the C terminus of human ER beta1
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry 1:5 - 1:50
  • Western Blot 1:500
• Application Notes: Positive control(s): Ovary
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Turtle, Goose

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ER beta/NR3A2 Antibody (PPG5/10) - BSA Free

• ER beta
• Erb
• ER-beta
• ESR2
• ESR-BETA
• ESTRB
• estrogen receptor 2 (ER beta)
• estrogen receptor beta 4
• estrogen receptor beta
• NR3A2
• NR3A2ESRB
• Nuclear receptor subfamily 3 group A member 2

Background

Estrogen receptors are commonly over-expressed in cancers. Clone PPG5/10 recognizes Estrogen Receptor Beta (ER beta) 1 and is useful in determining the expression of ER beta 1 in cancer cells, mainly via immunohistochemistry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER beta/NR3A2 Antibody (NBP2-61944)(2)

Publications using NBP2-61944

• Weber F, Schueler-Toprak S, Buechler C et al. Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling Diagnostics (Basel, Switzerland) 2023-03-02 [PMID: 36900088] (IHC-P, Human)
  
  Details:
  1:20 IHC-P
• Tantengco O, Richardson L, Mennon R Effects of a gestational level of estradiol on cellular transition, migration, and inflammation in cervical epithelial and stromal cells Am J Reprod Immunol 2020-11-05 [PMID: 33152143] (ICC/IF, Human)

Review for ER beta/NR3A2 Antibody (NBP2-61944) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 01/20/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: Endocervical epithelial cells
• Species: Human
• Lot: BSVEA190301

Comments

• Comments: 1:200 dilution in 3% BSA

Bioinformatics

• Gene Symbol: ESR2