ER beta/NR3A2 Antibody (733930) [Alexa Fluor® 488]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ER beta /NR3A2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7106G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 733930
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

ER beta/NR3A2 Antibody (733930) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human ER beta /NR3A2
  Met1-Gly318
  Accession # Q92731
• Specificity: Detects human ER beta /NR3A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ER alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 12 months, 2 to 8 °C under sterile conditions after opening.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ER beta/NR3A2 Antibody (733930) [Alexa Fluor® 488]

• ER beta
• Erb
• ER-beta
• ESR2
• ESR-BETA
• ESTRB
• estrogen receptor 2 (ER beta)
• estrogen receptor beta 4
• estrogen receptor beta
• NR3A2
• NR3A2ESRB
• Nuclear receptor subfamily 3 group A member 2

Background

Estrogen Receptor beta (ER beta ; also known as NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta ‑estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha  function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha . Both ER alpha and ER beta can give rise to numerous isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER beta/NR3A2 Antibody (IC7106G)(1)

Publication using IC7106G

• R Akyu Takei, K Tomihara, M Yamazaki, R Moniruzzam, W Heshiki, K Sekido, H Tachinami, K Sakurai, A Yonesi, S Imaue, K Fujiwara, M Noguchi Protumor role of estrogen receptor expression in oral squamous cell carcinoma cells Oral surgery, oral medicine, oral pathology and oral radiology, 2021-04-30;0(0):. 2021-04-30 [PMID: 34518137] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ESR2
• Uniprot:
  • Q92731()