Home » ER beta/NR3A2 » ER beta/NR3A2 Antibodies » ER beta/NR3A2 Antibody (14C8) [DyLight 350]

ER beta/NR3A2 Antibody (14C8) [DyLight 350]

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB200-305UV
    • Availability
      Product Discontinued

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ER beta/NR3A2 Antibody (14C8) [DyLight 350] Summary

Immunogen
Amino acids 1-153 of human ER-beta expressed in E. coli.
Specificity
Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ESR2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 4
using
NB200-305UV in the following applications:

Reactivity Notes

Rabbit reactivity reported in (PMID: 23664379 ). Mouse reactivity reported in scientific literature (PMID: 15750629). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ER beta/NR3A2 Antibody (14C8) [DyLight 350]

  • ER beta
  • Erb
  • ER-beta
  • ESR2
  • ESR-BETA
  • ESTRB
  • estrogen receptor 2 (ER beta)
  • estrogen receptor beta 4
  • estrogen receptor beta
  • NR3A2
  • NR3A2ESRB
  • Nuclear receptor subfamily 3 group A member 2

Background

The human ER-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ER beta/NR3A2 Antibody (NB200-305UV) (0)

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Review for ER beta/NR3A2 Antibody (NB200-305UV) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB200-305UV:
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(1)
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  4
reviewed by:
Verified Customer
 Flow Human 10/07/2013
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Summary

ApplicationFlow Cytometry
Sample Testedbreast cancer MCF7 cells
SpeciesHuman

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 Verified Customer
10/07/2013
Application: Flow
Species: Human
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Bioinformatics

Gene Symbol ESR2