Novus Biologicals products are now on bio-techne.com

Ephrin-A1 Overexpression Lysate

Images

 
Western Blot: Ephrin A1 Overexpression Lysate (Adult Normal) [NBP2-04832] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ephrin A1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Ephrin-A1 Overexpression Lysate Summary

Description

Ephrin-A1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC212122

Accession#: NM_182685

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
EFNA1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
19.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Ephrin-A1 Overexpression Lysate

  • B61
  • ECKLG
  • EFL1
  • EFL-1
  • EFNA1
  • EPH-related receptor tyrosine kinase ligand 1
  • EphrinA1
  • Ephrin-A1
  • EPLG1TNF alpha-induced protein 4
  • Immediate early response protein B61
  • LERK1
  • LERK1LERK-1
  • ligand of eph-related kinase 1
  • TNFAIP4B61
  • Tumor necrosis factor alpha-induced protein 4
  • tumor necrosis factor, alpha-induced protein 4

Background

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that Eph family members are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7); ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3035
Species: Hu
Applications: CyTOF-ready, Flow, IHC, KO, WB
AF638
Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
AF473
Species: Mu
Applications: IHC, Simple Western, WB
8415-A2
Species: Mu
Applications: BA
359-EA
Species: Hu
Applications: Bind
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
MAB369
Species: Hu
Applications: IHC, WB
NBP2-61871
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF641
Species: Mu
Applications: IHC, WB
DVE00
Species: Hu
Applications: ELISA
AF496
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, WB
NBP2-67150
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
NBP3-17255
Species: Hu
Applications: ICC/IF, WB
NBP1-59069
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF4467
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
374-EA
Species: Hu
Applications: Bind
H00003126-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-04832
Species: Hu
Applications: WB

Publications for Ephrin-A1 Lysate (NBP2-04832) (0)

There are no publications for Ephrin-A1 Lysate (NBP2-04832).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Ephrin-A1 Lysate (NBP2-04832) (0)

There are no reviews for Ephrin-A1 Lysate (NBP2-04832). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ephrin-A1 Lysate (NBP2-04832) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Ephrin-A1 Products

Research Areas for Ephrin-A1 Lysate (NBP2-04832)

Find related products by research area.

Blogs on Ephrin-A1

There are no specific blogs for Ephrin-A1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ephrin-A1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol EFNA1