ENT2 Overexpression Lysate (Native)

Images

 
Western Blot: ENT2 Overexpression Lysate (Adult Normal) [NBL1-16092] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ENT2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ENT2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210776 and based on accession number NM_001532. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2), mRNA.
Gene
SLC29A2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
49.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ENT2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ENT2 Overexpression Lysate (Native)

  • Delayed-early response protein 12
  • DER12Solute carrier family 29 member 2
  • ENT2
  • Equilibrative NBMPR-insensitive nucleoside transporter
  • Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleosidetransporter
  • equilibrative nucleoside transporter 2
  • HNP3636 kDa nucleolar protein HNP36
  • Hydrophobic nucleolar protein, 36 kDa
  • hydrophobic nucleolar protein, 36kD
  • Nucleoside transporter, ei-type
  • solute carrier family 29 (nucleoside transporters), member 2

Background

The uptake of nucleosides by transporters, such as SLC29A2, is essential for nucleotide synthesis by salvage pathways in cells that lack de novo biosynthetic pathways. Nucleoside transport also plays a key role in the regulation of many physiologic processes through its effect on adenosine concentration at the cell surface (Griffiths et al., 1997 [PubMed 9396714]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ENT2 Lysate (NBL1-16092) (0)

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Bioinformatics

Gene Symbol SLC29A2