Human ENPP-2/Autotaxin ELISA Kit (Colorimetric)

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Human ENPP-2/Autotaxin ELISA Kit (Colorimetric) [NBP3-26056] - Standard Curve Reference

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP3-26056
    • Availability
      Product Discontinued

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Human ENPP-2/Autotaxin ELISA Kit (Colorimetric) Summary

Description
This ELISA kit has been transitioned to a new version, available here: DENP20
Standard Curve Range
93.75 - 6000 pg/mL
Sensitivity
43.21 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Kit Type
ELISA Kit (Colorimetric)
Gene
ENPP2

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 100X Avidin-HRP Conjugate
  2. 100X ENPP-2/Autotaxin HRP-Detector Anitbody
  3. 30X Wash Buffer
  4. Conjugate Diluent
  5. Detector Antibody Diluent
  6. ENPP-2/Autotaxin Lyophilized Standard
  7. ENPP-2/Autotaxin Microplate
  8. Standard Diluent
  9. Stop Solution
  10. TMB Substrate

Alternate Names for Human ENPP-2/Autotaxin ELISA Kit (Colorimetric)

  • ATX
  • ATXFLJ26803
  • ATX-X
  • Autotaxin
  • autotaxin-t
  • EC 3.1.4.39
  • ectonucleotide pyrophosphatase/phosphodiesterase 2
  • ectonucleotide pyrophosphatase/phosphodiesterase family member 2
  • E-NPP 2
  • ENPP2
  • ENPP-2
  • Extracellular lysophospholipase D
  • Lysophosphatidic Acid
  • LysoPLD
  • NPP2
  • PD-IALPHA
  • PDNP2
  • PDNP2NPP2
  • phosphodiesterase I/nucleotide pyrophosphatase 2
  • plasma lysophospholipase D

Background

The protein encoded by the ENPP2 gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for ENPP-2/Autotaxin ELISA Kit (NBP3-26056). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol ENPP2