LPAR1/LPA1/EDG-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS212] - Skin, Melanoma

Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS212] - Brain, Glioblastoma

• Reactivity: Hu, Rt, Pm, Pm, Mu, Po, Av, Bt, Bv, Ca, Ch, Eq, Ha, Ma-Op, Rb, Sh
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LPAR1/LPA1/EDG-2 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 15 amino acid peptide from N-terminal extracellular domain of human LPAR1/LPA1/EDG-2.
• Epitope: N-Terminus extracellular domain
• Localization: Cell Membrane
• Specificity: Human EDG2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DDX60 (53%), ACER3 (47%).
• Predicted Species: Mouse (93%), Porcine (93%), Bovine (93%), Rabbit (93%), Opossum (93%), Avian (93%), Equine (93%), Canine (93%), Hamster (93%), Chicken (93%), Bat (93%), Sheep (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LPAR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 31546971)..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Xenopus (87%), Zebrafish (87%). Rat reactivity reported in (PMID: 23451264).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LPAR1/LPA1/EDG-2 Antibody - BSA Free

• EDG2
• edg-2
• EDG2Lysophosphatidic acid receptor Edg-2
• endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,
• GPCR26
• GPR26
• LPA receptor 1
• LPA1
• LPA-1
• LPA1vzg-1
• LPAR1
• lysophosphatidic acid receptor 1
• Mrec1.3
• rec.1.3
• ventricular zone gene 1
• VZG1
• vzg-1

Background

Edg2, a Lysophospholipid/Lysosphingolipid Receptor, mediates growth factor-like activities such as proliferation, platelet aggregation, and chemotaxis. Edg2 is involved in myelin formation or maintenance. It is activated by platelet-derived lysophosphatidic acid (LPA), a lipid metabolite present in serum that acts ubiquitously. Although alternate translation start codons and polyadenylation sites have been reported, the alternative splicing transcripts each encode identical proteins. Edg2 has been reported in most human tissues, and is especially abundant in brain cortical regions. ESTs have been isolated from bone, brain, breast, connective tissue, embryo, heart/melanocyte/uterus, lung, prostate, and uterus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LPAR1/LPA1/EDG-2 Antibody (NLS212)(3)

Publications using NLS212

• Harper K, Brochu-Gaudreau K, Saucier C, M Dubois C Hypoxia Downregulates LPP3 and Promotes the Spatial Segregation of ATX and LPP1 During Cancer Cell Invasion Cancers (Basel) 2019-09-19 [PMID: 31546971] (ICC/IF, Human)
• Arriazu R, Duran E, Pozuelo JM, Santamaria L. Expression of Lysophosphatidic Acid Receptor 1 and Relation with Cell Proliferation, Apoptosis, and Angiogenesis on Preneoplastic Changes Induced by Cadmium Chloride in the Rat Ventral Prostate. PLoS One 2013-01-01 [PMID: 23451264] (IHC-P, Rat)
• Horak, CE et al. Nm23-H1 Suppresses Metastasis by Inhibiting Expression of the Lysophosphatidic Acid Receptor EDG2. Cancer Res 67(24): 11751-11759. 2007-01-01 [PMID: 18089805]

Bioinformatics

• Gene Symbol: LPAR1
• Entrez:
  • 1902(Human)
• Uniprot:
  • Q92633()