Human ENPP-2/Autotaxin Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human ENPP-2/Autotaxin Quantikine ELISA Kit Summary

• Background: The Quantikine Human ENPP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ENPP-2 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human ENPP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ENPP-2 showed linear curves that were parallel to the standard curv...es obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ENPP-2.
  Show More
• Specificity: Natural and recombinant human ENPP-2
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ENPP2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human ENPP-2/Autotaxin Quantikine ELISA Kit

• ATX
• ATXFLJ26803
• ATX-X
• Autotaxin
• autotaxin-t
• EC 3.1.4.39
• ectonucleotide pyrophosphatase/phosphodiesterase 2
• ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• E-NPP 2
• ENPP2
• ENPP-2
• Extracellular lysophospholipase D
• Lysophosphatidic Acid
• LysoPLD
• NPP2
• PD-IALPHA
• PDNP2
• PDNP2NPP2
• phosphodiesterase I/nucleotide pyrophosphatase 2
• plasma lysophospholipase D

Background

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain. Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine. The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site. Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for ENPP-2/Autotaxin (DENP20)(6)

Publications using DENP20

• Schmithals, C;Kakoschky, B;Denk, D;von Harten, M;Klug, JH;Hintermann, E;Dropmann, A;Hamza, E;Jacomin, AC;Marquardt, JU;Zeuzem, S;Schirmacher, P;Herrmann, E;Christen, U;Vogl, TJ;Waidmann, O;Dooley, S;Finkelmeier, F;Piiper, A; Tumour-specific activation of a tumour-blood transport improves the diagnostic accuracy of blood tumour markers in mice EBioMedicine 2024-06-17 [PMID: 38889481] (Mouse)
• Pietrobono, S;Sabbadini, F;Bertolini, M;Mangiameli, D;De Vita, V;Fazzini, F;Lunardi, G;Casalino, S;Scarlato, E;Merz, V;Zecchetto, C;Quinzii, A;Di Conza, G;Lahn, M;Melisi, D; Autotaxin secretion is a stromal mechanism of adaptive resistance to TGF? inhibition in pancreatic ductal adenocarcinoma Cancer research 2023-09-22 [PMID: 37738399] (Human)
• FM Trovato, S Mujib, E Jerome, A Cavazza, P Morgan, J Smith, MT Depante, K O'Reilly, J Luxton, T Mare, S Napoli, MJ McPhail Immunometabolic analysis shows a distinct cyto-metabotype in Covid-19 compared to sepsis from other causes Heliyon, 2022-06-24;8(6):e09733. 2022-06-24 [PMID: 35774516] (Human)
• Pleli T, Martin D, Kronenberger B, Brunner F, Koberle V, Grammatikos G, Farnik H, Martinez Y, Finkelmeier F, Labocha S, Ferreiros N, Zeuzem S, Piiper A, Waidmann O Serum autotaxin is a parameter for the severity of liver cirrhosis and overall survival in patients with liver cirrhosis--a prospective cohort study. PLoS ONE, 2014-07-25;9(7):e103532. 2014-07-25 [PMID: 25062038] (Human)
• Nakao , Momoko, Sugaya , Makoto, Suga , Hiraku, Kawaguchi , Makiko, Morimura , Sohshi, Kai , Hiromich, Ohmatsu , Hanako, Fujita , Hideki, Asano , Yoshihid, Tada , Yayoi, Kadono , Takafumi, Sato , Shinichi Serum autotaxin levels correlate with pruritus in patients with atopic dermatitis. J Invest Dermatol, 2014-01-17;134(6):1745-7. 2014-01-17 [PMID: 24441098] (Human)
• Madan , Damian, Ferguson , Colin G, Lee , Won Yong, Prestwich , Glenn D, Testa , Charles Non-invasive imaging of tumors by monitoring autotaxin activity using an enzyme-activated near-infrared fluorogenic substrate. PLoS ONE, 2013-11-20;8(11):e79065. 2013-11-20 [PMID: 24278115] (Human)

Bioinformatics

• Gene Symbol: ENPP2