Endomucin/Mucin -14 Antibody

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Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7206) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Endomucin/Mucin -14 Antibody Summary

Immunogen
E. coli-derived recombinant human Endomucin
Asn19-Gly139
Accession # Q9ULC0
Specificity
Detects human Endomucin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Endomucin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EMCN
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endomucin/Mucin -14 Antibody

  • EMCN
  • EMCN2
  • Endomucin
  • MUC-14

Background

Endomucin (Endothelial sialomucin; also MUC-14, Endomucin-2 and Ga34) is an 85-115 kDa glycoprotein that belongs to no known family of molecules. It is expressed by primitive bone marrow stem cells, endothelial cells of both blood and lymphatic vessels, and select keratinocytes. Endomucin appears to create an environment that discourages leukocyte binding to vascular walls, and yet may also serve as an L-Selectin ligand. Mature human Endomucin is a 243 amino acid (aa) type I transmembrane glycoprotein (aa 19-261). It contains a 172 aa extracellular domain (ECD) (aa 19-190) plus a 50 aa cytoplasmic tail (aa 212-261). The ECD possesses a uteroglobin-like domain (aa 19-74) plus multiple sites for N- and O-linked glycosylation. There are potential splice variants that seem to parallel those in mouse. One shows a deletion of aa 140-222 and is likely soluble, while another possesses a Ser substitution for aa 126-139. Over aa 19-139, human Endomucin shares only 35% aa identity with mouse Endomucin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EMCN
Uniprot