Emerin Antibody (CL0201) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line RT-4

Staining of human fallopian tube shows strong positivity in the nuclear membrane of glandular cells.

Staining of human kidney shows strong immunoreactivity in the nuclear membrane in renal glomeruli and tubuli.

Staining of human placenta shows strong immunoreactivity in the nuclear membrane in the trophoblast layer.

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-Emerin antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Staining of human stomach shows strong positivity in the nuclear membrane of glandular cells.

Staining of PC-3 cells using the Anti-Emerin monoclonal antibody) .

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Emerin Antibody (CL0201) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Emerin Antibody (CL0201) - Azide and BSA Free (NBP2-52876) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P50402, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EMD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Emerin Antibody (CL0201) - Azide and BSA Free

• emerin
• Emery-Dreifuss muscular dystrophy
• LEM domain containing 5
• STAEDMDLEMD5

Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EMD