Emerin Antibody (CL0201) - BSA Free

Western Blot: Emerin Antibody (CL0201) [NBP2-52876] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Western Blot: Emerin Antibody (CL0201) [NBP2-52876] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0201) [NBP2-52876] - Staining of PC-3 cells using the Anti-EMD monoclonal antibody, showing specific staining in the nuclear membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human stomach shows strong positivity in the nuclear membrane of glandular cells.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human fallopian tube shows strong positivity in the nuclear membrane of glandular cells.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human kidney shows strong immunoreactivity in the nuclear membrane in renal glomeruli and tubuli.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human placenta shows strong immunoreactivity in the nuclear membrane in the trophoblast layer.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL0201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Emerin Antibody (CL0201) - BSA Free Summary

• Description: Novus Biologicals Mouse Emerin Antibody (CL0201) - BSA Free (NBP2-52876) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Emerin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P50402, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EMD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  Emerin Recombinant Protein Antigen (NBP2-52876PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Emerin Antibody (CL0201) - BSA Free

• emerin
• Emery-Dreifuss muscular dystrophy
• LEM domain containing 5
• STAEDMDLEMD5

Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Emerin Antibody (NBP2-52876)(3)

Publications using NBP2-52876

• Hiroe T, Moriya S, Kobayashi S et al. Negative correlation between the nuclear size and nuclear Lamina component Lamin A in intraductal papillary mucinous neoplasms of the pancreas Pathology oncology research : POR 2022-12-06 [PMID: 36561231] (IHC-P, Human)
• Watabe S, Kobayashi S, Hatori M Et al. Role of Lamin A and emerin in maintaining nuclear morphology in different subtypes of ovarian epithelial cancer Oncology letters 2022-01-01 [PMID: 34820008] (IHC-P, Human)
• Kobayashi S, Saio M, Fukuda T et al. Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma. Oncol Rep. 2018-11-02 [PMID: 30542735] (IHC-P, Human)

Bioinformatics

• Gene Symbol: EMD