ELTD1/ETL Antibody - BSA Free

Immunohistochemistry-Paraffin: ELTD1/ETL Antibody - BSA Free [NBP2-29387] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: ELTD1/ETL Antibody - BSA Free [NBP2-29387] - Analysis of anti-ELTD1 antibody with human colon, smooth muscle at 10 ug/ml.

• Reactivity: Hu, Pm, Pm
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ELTD1/ETL Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 19 amino acid peptide from N-terminal extracellular domain of human ELTD1/ETL.
• Epitope: N-Terminus extracellular domain
• Specificity: Human ELTD1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZNF646 (37%).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADGRL4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
• Application Notes: IHC: NBP2-29387 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Rabbit (89%), Canine (84%), Equine (84%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ELTD1/ETL Antibody - BSA Free

• EGF, latrophilin and seven transmembrane domain containing 1
• EGF, latrophilin and seven transmembrane domain-containing protein 1
• ELTD1
• ETL protein
• ETL
• ETLEGF-TM7-latrophilin-related protein
• KPG_003

Background

The ETL protein receptor has been characterized as an Orphan-B GPCR with an unknown ligand. ETL has a large extracellular domain with epidermal growth factor (EGF)-like repeats, a seven-transmembrane domain, and a short cytoplasmic tail. ETL expression is developmentally regulated in heart. ETL protein receptor expression has been documented in heart, lung, and vessel. ESTs have been isolated from ear, embryo, heart, liver/spleen, lung, pineal gland, placenta, prostate, skeletal muscle, testis, and vessel libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADGRL4
• Uniprot:
  • Q9HBW9(Human)