Elk-1 Antibody (7E10D5)

Western Blot: Elk-1 Antibody (7E10D5) [NB110-60513] - Analysis using anti-ELK1 monoclonal antibody against truncated ELK1 recombinant protein(1) and K562 cell lysate(2).

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 7E10D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Elk-1 Antibody (7E10D5) Summary

• Description: Novus Biologicals Mouse Elk-1 Antibody (7E10D5) (NB110-60513) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of Elk-1 expressed in E. Coli.
• Specificity: ELK1 (7E10D5)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ELK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Elk-1 Antibody (7E10D5)

• Elk1
• Elk-1
• ELK1, member of ETS oncogene family
• ETS domain-containing protein Elk-1
• ETS-like gene 1

Background

The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELK1
• Entrez:
  • 2002(Human)