eIF5A2 Antibody (OTI6G7) - Azide and BSA Free

Western Blot: eIF5A2 Antibody (OTI6G7) - Azide and BSA Free [NBP2-72056] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF5A2.

Immunocytochemistry/Immunofluorescence: eIF5A2 Antibody (OTI6G7) - Azide and BSA Free [NBP2-72056] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY EIF5A2.

Immunohistochemistry: eIF5A2 Antibody (OTI6G7) - Azide and BSA Free [NBP2-72056] - Analysis of Human pancreas tissue.

Flow Cytometry: eIF5A2 Antibody (OTI6G7) - Azide and BSA Free [NBP2-72056] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by eIF5A2 antibody.

Immunohistochemistry: eIF5A2 Antibody (OTI6G7) - Azide and BSA Free [NBP2-72056] - Analysis of Carcinoma of Human pancreas tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI6G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

eIF5A2 Antibody (OTI6G7) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human EIF5A2(NP_065123) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF5A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 16.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for eIF5A2 Antibody (OTI6G7) - Azide and BSA Free

• EIF-5A2
• eIF-5A-2
• eIF5AII
• Eukaryotic initiation factor 5A isoform 2
• eukaryotic initiation factor 5A
• eukaryotic translation initiation factor 5A2
• eukaryotic translation initiation factor 5A-2

Background

Eukaryotic translation initiation factor 5A (eIF5A) is an essential protein tightly linked to cellular polyamine homeostasis. EIF5A2, a novel phylogenetically conserved gene for eIF5A islocalized in an area of the genome often noted for chromosomal instability in cancers. EIF5A2 expression has been observed in testis, brain and colorectal adenocarcinoma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5A2