EIF5 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP3-00231] - Immunochemical staining of human EIF5 in human breast carcinoma with NBP3-00231 (1:500, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP3-00231] - Immunochemical staining of human EIF5 in human colon carcinoma with NBP3-00231 (1:300, formalin-fixed paraffin embedded sections).

Immunocytochemistry/ Immunofluorescence: EIF5 Antibody [NBP3-00231] - Immunofluorescence staining of EIF5 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00231 (1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

• Reactivity: Hu
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EIF5 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human EIF5 (Accession#: P55010; Met1-Asp150)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF5
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for EIF5 Antibody - Azide and BSA Free

• eIF-5
• EIF-5A
• eukaryotic translation initiation factor 5

Background

EIF5 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingos

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5