eIF3X Antibody

Western Blot: eIF3X Antibody [NB100-93306] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibody: Affinity purified rabbit anti-eIF3X antibody NB100-93306 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF3X was also immunoprecipitated by rabbit anti-eIF3X antibody NB100-93305, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3X, the ReliaBLOT (R) Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

eIF3X Antibody Summary

• Description: Novus Biologicals Rabbit eIF3X Antibody (NB100-93306) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-eIF3X Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1259 and 1309 of human putative eukaryotic translation initiation factor 3 subunit using the numbering given in entry NP_056044.3 (GeneID 23277).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLUH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:32149416).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25349259).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF3X Antibody

• CLU1
• DKFZp686L05242
• DKFZp686M09233
• FLJ23672
• hypothetical protein LOC23277
• KIAA0664

Background

eIF3X (putative eukaryotic translation initiation factor 3 subunit) belongs to the eIF-3 p135 family of evolutionarily conserved eIF-3 135kDa subunits found in yeast, nematode, slime mold, and human cells. In yeast, eIF3 p135 is not part of the core eIF-3 complex, but is proposed to be a probable component with unknown function

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF3X Antibody (NB100-93306)(6)

Publications using NB100-93306

• Marta Zaninello, Tim Schlegel, Hendrik Nolte, Mujeeb Pirzada, Elisa Savino, Esther Barth, Ines Klein, Hauke Wüstenberg, Tesmin Uddin, Lisa Wolff, Brunhilde Wirth, Helmar C. Lehmann, Jean-Michel Cioni, Thomas Langer, Elena I. Rugarli CLUH maintains functional mitochondria and translation in motoneuronal axons and prevents peripheral neuropathy Science Advances 2024-05-31 [PMID: 38809982]
• Schatton D, Di Pietro G, Szczepanowska K et al. CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression eLife 2022-05-13 [PMID: 35559794] (Western Blot, Human, Mouse)
• HEmono M, Haller A, Chicher J et al. The interactome of CLUH reveals its association to SPAG5 and its co-translational proximity to mitochondrial proteins BMC biology 2022-01-10 [PMID: 35012549] (RIP, ICC/IF, WB, Mouse, Human)
• Pla-Martin D, Schatton D, Wiederstein Jl et Al. CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy EMBO J. 2020-03-09 [PMID: 32149416] (WB, ICC/IF, Mouse)
• Wakim J, Goudenege D et al. CLUH couples mitochondrial distribution to the energetic and metabolic status. J Cell Sci 2017-01-06 [PMID: 28424233] (WB, Human)
• Gao J, Schatton D, Martinelli P et al. CLUH regulates mitochondrial biogenesis by binding mRnAs of nuclear-encoded mitochondrial proteins. CLUH regulates mitochondrial biogenesis by binding mRnAs of nuclear-encoded mitochondrial proteins. 2014-10-27 [PMID: 25349259] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: CLUH
• Entrez:
  • 23277(Human)
• Uniprot:
  • O75153(Human)