EIF3J Antibody

Images

 
Western Blot: EIF3J Antibody [NB100-93287] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-93287 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) ...read more
Immunohistochemistry-Paraffin: EIF3J Antibody [NB100-93287] - Human prostate carcinoma (left) and mouse teratoma (right). Antibody used at a dilution of 1:1,000.
Immunohistochemistry-Paraffin: EIF3J Antibody [NB100-93287] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-eIF3J/EIF3S1 used at a dilution of 1:500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

EIF3J Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 208 and 258 of human eukaryotic translation initiation factor 3, subunit J (eukaryotic translation initiation factor 3, subunit 1) using the numbering given in entry NP_003749.2 (G
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3J
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Positive Control
EIF3J Lysate (NBL1-10198)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF3J Antibody

  • eIF3 p35
  • eIF3-alpha
  • eIF3j
  • eIF3-p35
  • EIF3S1eIF-3-alpha
  • Eukaryotic translation initiation factor 3 subunit 1
  • eukaryotic translation initiation factor 3 subunit J
  • eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
  • eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa
  • eukaryotic translation initiation factor 3, subunit J

Background

Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004 [PubMed 14688252]; Fraser et al., 2007 [PubMed 17588516]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF3J
Entrez
Uniprot