EIF3A Antibody

Western Blot: EIF3A Antibody [NBP2-24570] - analysis of eIF3A in Daudi lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 lysate using eIF3A antibody at 0.5 ug/ml, 0.5 ug/ml and 0.25 ug/ml respectively.

Immunohistochemistry-Paraffin: EIF3A Antibody [NBP2-24570] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3A antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Simple Western: EIF3A Antibody [NBP2-24570] - Simple Western lane view shows a specific band for EIF3A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Discontinued

EIF3A Antibody Summary

• Immunogen: A portion of amino acids 550-600 of human eIF3A was used as the immunogen for the antibody.
• Predicted Species: Rat (100%), Chicken (100%), Canine (100%), Primate (100%), Equine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Simple Western 1:50
  • Western Blot 0.25-1.0 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EIF3A Antibody

• centrosomin homolog
• cytoplasmic protein p167
• eIF3 p167
• eIF3 p180
• eIF3 p185
• EIF3, p180 subunit
• eIF3a
• eIF3-p170
• EIF3S10EIF3
• eIF-3-theta
• eIF3-theta
• Eukaryotic translation initiation factor 3 subunit 10
• eukaryotic translation initiation factor 3 subunit A
• eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD)
• eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD)
• eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa
• eukaryotic translation initiation factor 3, subunit 10, 170kD
• eukaryotic translation initiation factor 3, subunit A
• KIAA0139P167
• p180
• p185
• TIF32

Background

Eukaryotic initiation factor 3 subunit A (eIF3A) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3A is also known as EIF3S10 (eukaryotic initiation factor 3 subunit 7). eIF3A is the largest subunit of the eIF3 complex. eIF3A expression is regulated through the cell cycle. It's expression peaks in S-phase and it is proposed to play a role in regulating the translation of mRNAs involved in the regulation of cell cycle progression and proliferation. Alternative names for eIF3A include eIF-3-theta, eIF-3 p167, eIF-3 p170, eIF-3 p180, eIF3-p185, EIF3S10, and KIAA0139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF3A
• Uniprot:
  • Q14152(Human)